In situ binding assay to detect Myosin-1c interactions with hair-cell proteins.
In situ binding assay to detect Myosin-1c interactions with hair-cell proteins.
复制标题
原位结合测定检测 Myosin-1c 与毛细胞蛋白的相互作用。
DOI：
10.1007/978-1-59745-490-2_8
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Cyr,JanetL
中科院分区：
文献类型：
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作者：
Phillips,KelliR;Cyr,JanetL
肌球蛋白-1c是一种非传统的肌球蛋白，参与毛细胞机械转导，这一过程是我们听力和平衡的基础。为了研究肌球蛋白-1c与毛细胞转导复合体的其他成分的相互作用，我们开发了一种原位结合试验，可以显示肌球蛋白-1c与毛细胞蛋白的结合。在本章中，我们详细描述了表达和纯化重组肌球蛋白-1c片段所需的方法，以及它们在原位结合分析中的应用。
Myosin-1c is an unconventional myosin involved in hair-cell mechanotransduction, a process that underlies our senses of hearing and balance. To study the interaction of myosin-1c with other components of the hair-cell transduction complex, we have developed an in situ binding assay that permits visualization of myosin-1c binding to hair-cell proteins. In this chapter we describe in detail the methods needed for the expression and purification of recombinant myosin-1c fragments and their use in the in situ binding assay.