GASSHO1 and GASSHO2 encoding a putative leucine-rich repeat transmembrane-type receptor kinase are essential for the normal development of the epidermal surface in Arabidopsis embryos.
GASSHO1 and GASSHO2 encoding a putative leucine-rich repeat transmembrane-type receptor kinase are essential for the normal development of the epidermal surface in Arabidopsis embryos.
复制标题
DOI：
10.1111/j.1365-313x.2007.03395.x
复制
发表时间：
2007-12
期刊：
影响因子：
--
通讯作者：
Ryo Tsuwamoto;H. Fukuoka;Y. Takahata
中科院分区：
文献类型：
--
作者：
Ryo Tsuwamoto;H. Fukuoka;Y. Takahata
受体样激酶（Receptor-like kinases，RLK）是一类富含亮氨酸重复序列（leucine-rich repeats，LRR）的蛋白质，在配体介导的细胞间通讯中既是信号受体又是信号转导子。据信，许多LRR-RLKs存在于拟南芥基因组中，但大多数的功能是未知的。我们最近鉴定了Bnms 4D-82，一个在甘蓝型油菜中编码LRR-RLK的表达序列标签（EST），并且在其小孢子胚胎发生的早期阶段表达。为了阐明该基因的功能，我们使用GASSHO 1（GSO 1）和GSO 2，这两个拟南芥基因与Bnms 4D-82具有高度同源性。GSO 1和GSO 2的转录产物在胚和幼苗中积累，但在真叶中不积累。缺乏GSO 1和GSO 2的植物表现出多效性，包括胚胎的异常弯曲，子叶之间的异位粘附，高渗透性的表皮结构，以及胚胎和幼苗中子叶上气孔分布的异常模式。然而，无论是gso 1 -1或gso 2 -1纯合的植物没有明显的异常。这些结果表明，GASSHO基因是胚胎发育过程中形成正常表皮表面所必需的。
Receptor-like kinases (RLKs) containing leucine-rich repeats (LRRs) act as both signal receptor and signal transducer in ligand-mediated communication between cells. It is believed that many LRR-RLKs are present in the Arabidopsis genome, but the functions of most are unknown. We recently identified Bnms4D-82, an expressed sequence tag (EST) in Brassica napus that encodes an LRR-RLK and is expressed at an early stage of its microspore embryogenesis. To elucidate the function of this gene we used GASSHO1 (GSO1) and GSO2, two Arabidopsis genes with a high degree of homology with Bnms4D-82. The products of transcripts of GSO1 and GSO2 accumulate in parts of the embryo and in seedlings, but not in true leaves. Plants that lacked both GSO1 and GSO2 exhibited pleiotropy, including abnormal bending of embryos, ectopic adhesion between cotyledons, a highly permeable epidermal structure, and an abnormal pattern of distribution of stomata on cotyledons in embryos and seedlings. However, plants homozygous for either gso1-1 or gso2-1 had no visible abnormality. These results suggest that GASSHO genes are essential for the formation of a normal epidermal surface during embryogenesis.