Electron microscopic analysis of replicating DNA of sea urchin embryos
Electron microscopic analysis of replicating DNA of sea urchin embryos
复制标题
海胆胚胎复制 DNA 的电子显微镜分析
DOI：
10.1016/0092-8674(78)90293-3
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发表时间：
1978
期刊：
影响因子：
64.5
通讯作者：
Mario Buonglorno
中科院分区：
文献类型：
--
作者：
C. Baldari;F. Amaldi;Mario Buonglorno
从受精后第3 S期卵黄旁胚中提取DNA，用电镜对其进行分析。在这种积极复制的DNA中观察到的最相关的结构是小簇，紧密间隔的微泡（平均约0.1@ rn长），部分或完全单链分子和少数线性分叉。出乎意料的是，没有观察到长眼睛。从原肠胚和成人体细胞组织中纯化的DNA分析显示出相同的结构，尽管频率较低。通过定量分析确定了微气泡的尺寸分布和间距。为了更好地表征这些结构，已经进行了许多控制实验。讨论了各种可能性，以解释观察到的形式的存在和没有大眼睛。
DNA was extracted from Paracentrotus lividus embryos at the third S phase after fertilization and analyzed with the electron microscope. The most relevant structures observed in this actively replicating DNA are clusters of shoti, closely spaced microbubbles (about 0.1@ rn long on the average), partially or entirely single-stranded molecules and few linear forks. Unexpectedly, no long eye forms were observed. The analysis of DNA purified from gastrulae and from adult somatic tissues has revealed the same structures, although at a low frequency. A quantitative analysis has been carried out to determine the size distribution and spacing of microbubbles. A number of control experiments have been performed to characterize these structures better. Various possibilities are discussed to account for the presence of the observed forms and the absence of larger eyes.