Epithelial Membrane Protein-2 in Human Proliferative Vitreoretinopathy and Epiretinal Membranes.
Epithelial Membrane Protein-2 in Human Proliferative Vitreoretinopathy and Epiretinal Membranes.
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DOI：
10.1167/iovs.15-17791
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发表时间：
2016-06-01
影响因子：
4.4
通讯作者：
Gordon LK
中科院分区：
文献类型：
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作者：
Telander DG;Yu AK;Forward KI;Morales SA;Morse LS;Park SS;Gordon LK
目的检测增生性玻璃体视网膜病变（PVR）或视网膜前膜（ERMs）手术患者视网膜前膜中上皮膜蛋白2（EMP 2）的表达水平。EMP 2是一种整合素调节剂，在视网膜色素上皮细胞中表达，了解EMP 2在人类视网膜疾病中的表达可能有助于确定EMP 2是否是潜在的治疗靶点。在获得同意后，在玻璃体切除术期间收集视网膜前膜。将膜固定、处理、切片，并通过免疫组织化学评价EMP 2的蛋白表达。由蒙眼观察者比较膜中染色强度（SI）和阳性细胞百分比（PP）。根据其原因和类型（包括炎症和创伤性）对膜进行分类。所有的膜对EMP 2染色呈阳性。炎症性玻璃体视网膜病变诱导的膜（所有原因）显示EMP 2的表达高于ERMs，具有较高的SI（1.81 vs. 1.38; P = 0.07）和PP（2.08 vs. 1.54; P = 0.09）。然而，所有PVR亚组具有相似的EMP 2表达水平，通过Kruskal-Wallis检验没有统计学显著差异。炎性PVR的EMP 2表达高于ERM（SI为2.58 vs. 1.38）;然而，这并不具有统计学显著性。EMP 2表达与PVR膜形成时间无相关性。通过RT-PCR在所有测试样品（n = 6）中检测到EMP 2。所有研究的ERMs和PVR膜均表达EMP 2。EMP 2水平在所有PVR亚组中均高于ERM，尤其是在炎症性和创伤性PVR中。未来的研究需要确定EMP 2在包括PVR在内的各种视网膜疾病的发病机制和治疗中的作用。
To determine the level of epithelial membrane protein-2 (EMP2) expression in preretinal membranes from surgical patients with proliferative vitreoretinopathy (PVR) or epiretinal membranes (ERMs). EMP2, an integrin regulator, is expressed in the retinal pigment epithelium and understanding EMP2 expression in human retinal disease may help determine whether EMP2 is a potential therapeutic target. Preretinal membranes were collected during surgical vitrectomies after obtaining consents. The membranes were fixed, processed, sectioned, and protein expression of EMP2 was evaluated by immunohistochemistry. The staining intensity (SI) and percentage of positive cells (PP) in membranes were compared by masked observers. Membranes were categorized by their cause and type including inflammatory and traumatic. All of the membranes stained positive for EMP2. Proliferative vitreoretinopathy–induced membranes (all causes) showed greater expression of EMP2 than ERMs with higher SI (1.81 vs. 1.38; P = 0.07) and PP (2.08 vs. 1.54; P = 0.09). However all the PVR subgroups had similar levels of EMP2 expression without statistically significant differences by Kruskal-Wallis test. Inflammatory PVR had higher expression of EMP2 than ERMs (SI of 2.58 vs. 1.38); however, this was not statistically significant. No correlation was found between duration of PVR membrane and EMP2 expression. EMP2 was detected by RT-PCR in all samples (n = 6) tested. All studied ERMs and PVR membranes express EMP2. Levels of EMP2 trended higher in all PVR subgroups than in ERMs, especially in inflammatory and traumatic PVR. Future studies are needed to determine the role of EMP2 in the pathogenesis and treatment of various retinal conditions including PVR.