NORMAL EMBRYO FIBROBLASTS RELEASE TRANSFORMING GROWTH-FACTORS IN A LATENT FORM
NORMAL EMBRYO FIBROBLASTS RELEASE TRANSFORMING GROWTH-FACTORS IN A LATENT FORM
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DOI：
10.1002/jcp.1041210123
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发表时间：
1984-01-01
影响因子：
5.6
通讯作者：
JULLIEN, P
中科院分区：
文献类型：
--
作者：
LAWRENCE, DA;PIRCHER, R;JULLIEN, P
正常鸡、小鼠和人胚胎成纤维细胞以潜伏形式释放转化生长因子（TGF）到其培养基中。它们对2种广泛使用的靶细胞（大鼠NRK-49 F和小鼠AKR-2B）的软琼脂集落形成活性基本上仅在细胞条件培养基预先酸化后才显示。当在NRK-49 F细胞上测定时，这些TGF是EGF[表皮生长因子]依赖性的，而在AKR-2B细胞上是非EGF依赖性的。来自鸡源的TGF活性以500 kd[千道尔顿]、125 kd和20 kd的3种（表观）MW形式释放。
Normal chicken, mouse and human embryo fibroblasts release into their culture media transforming growth factors (TGF) in a latent form. Their soft agar colony-forming activity on 2 widely used target cells, rat NRK-49F and mouse AKR-2B, is essentially revealed only after prior acidification of cell-conditioned media. These TGF are EGF[epidermal growth factor]-dependent when assayed on NRK-49F cells and EGF-independent on AKR-2B cells. The TGF activity from the chicken source is released in 3 (apparent) MW forms of 500 kd[kilodalton], 125 kd and 20 kd.