Assigning protein functions by comparative genome analysis: Protein phylogenetic profiles
Assigning protein functions by comparative genome analysis: Protein phylogenetic profiles
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DOI：
10.1073/pnas.96.8.4285
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发表时间：
1999-04-13
影响因子：
11.1
通讯作者：
Yeates, TO
中科院分区：
文献类型：
--
作者：
Pellegrini, M;Marcotte, EM;Yeates, TO
从基因组序列中确定蛋白质功能是生物信息学的核心目标，我们提出了一种基于以下假设：在途径或结构复合物中一起发挥作用的蛋白质可能以相关方式发展。在进化过程中，所有此类功能连接的蛋白质倾向于在新物种中保存或消除，我们通过通过其系统发育特征表征每个蛋白来描述这种相关进化的特性，该字符串编码了每个已知的蛋白质的存在或不存在基因组。我们表明，具有匹配或类似特征的蛋白质倾向于在功能上链接。这种系统发育分析方法使我们能够预测未表征的蛋白质的功能。
Determining protein functions from genomic sequences is a central goal of bioinformatics, We present a method based on the assumption that proteins that function together in a pathway or structural complex are likely to evolve in a correlated fashion. During evolution, all such functionally linked proteins tend to be either preserved or eliminated in a new species, We describe this property of correlated evolution by characterizing each protein by its phylogenetic profile, a string that encodes the presence or absence of a protein in every known genome. We show that proteins having matching or similar profiles strongly tend to be functionally linked. This method of phylogenetic profiling allows us to predict the function of uncharacterized proteins.