The Protective Effect of Cu/Zn-SOD Against Oxidative Stress After PTEN Deletion
The Protective Effect of Cu/Zn-SOD Against Oxidative Stress After PTEN Deletion
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DOI：
10.3109/07357907.2011.554478
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发表时间：
2011-04
影响因子：
2.4
通讯作者：
Changhong Yu;Peilin Wang;Sheng Li;Xiu-hai Wang;Zhenlei Yu;Zhenhua Wang
中科院分区：
文献类型：
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作者：
Changhong Yu;Peilin Wang;Sheng Li;Xiu-hai Wang;Zhenlei Yu;Zhenhua Wang
活性氧（ROS）、抗氧化酶与癌变相互关联。探讨这些因子是否参与第十号染色体缺失的磷酸酶和张力蛋白同源物（PTEN）抑制肿瘤的机制。进行荧光探针和分子杂交以测量缺乏PTEN的细胞中的ROS和抗氧化酶水平。在通过PTEN缺失诱导氧化应激后，我们观察到抗氧化酶Cu/Zn-SOD的表达增加。此外，细胞的整体抗氧化能力减弱。这些结果表明，负调控发生通过ROS，Cu/Zn-SOD，和PTEN之间的相互作用，这种氧化和抗氧化之间的不平衡可能有助于减少在PTEN null细胞的抗氧化能力。这些研究将指导进一步研究PTEN的生物学功能。
Reactive oxygen species (ROS), antioxidant enzyme, and cancerization are related to each other. To investigate whether these factors are involved in the mechanism by which phosphatase and tensin homolog deleted on chromosome ten (PTEN) suppresses tumors. Fluorescent probes and molecular hybridizations were performed to measure ROS and antioxidant enzyme levels in cells lacking PTEN. After induction of oxidative stress by PTEN deletion, we observed an increase in the expression of the antioxidant enzyme Cu/Zn-SOD. In addition, the overall antioxidative capability of the cells was attenuated. These results suggest that negative regulation occurs through interactions among ROS, Cu/Zn-SOD, and PTEN, and such a disequilibrium between oxidation and antioxidation could contribute to the reduced antioxidative capacity in PTEN null cells. These studies will guide further investigations into the biological function of PTEN.