Penile alterations at early stage of type 1 diabetes in rats.
Penile alterations at early stage of type 1 diabetes in rats.
复制标题
DOI：
10.1590/s1677-5538.ibju.2016.0454
复制
发表时间：
2017-07
期刊：
影响因子：
--
通讯作者：
Liu G
中科院分区：
文献类型：
--
作者：
Tao M;Tasdemir C;Tasdemir S;Shahabi A;Liu G
糖尿病严重影响勃起功能。然而，在实验动物模型中，糖尿病早期阶段的阴茎改变还没有得到很好的研究。我们研究了糖尿病大鼠阴茎及其主要勃起成分的变化。 将雄性Sprague-Dawley大鼠分为2组：链脲佐菌素（STZ）诱导的糖尿病组和年龄匹配的对照组。糖尿病诱导后3周或9周，取出阴茎进行阴茎中段组织中平滑肌和神经元型一氧化氮合酶（nNOS）的免疫组织化学染色。对整个阴茎中段和阴茎海绵体的横截面积进行量化。定量海绵体中的平滑肌和背神经中的nNOS。 糖尿病患者的阴茎重量较低，但长度没有降低。糖尿病大鼠的总阴茎中段和阴茎海绵体的横截面积低于对照组9周，但不是3周后糖尿病诱导。糖尿病大鼠阴茎海绵体平滑肌横截面积占阴茎海绵体总面积的百分比低于对照组9周，但糖尿病诱导后3周不低于对照组。糖尿病大鼠背神经nNOS的百分比变化在3周时与对照组相似，在9周时与对照组相比有下降趋势。 糖尿病导致阴茎的时间改变，STZ大鼠模型中的显著变化在诱导后开始3-9周开始。需要进一步研究观察到的变化的可逆性。
Diabetes affects the erectile function significantly. However, the penile alterations in the early stage of diabetes in experimental animal models have not been well studied. We examined the changes of the penis and its main erectile components in diabetic rats. Male Sprague-Dawley rats were divided into 2 groups: streptozotocin (STZ)-induced diabetics and age-matched controls. Three or nine weeks after diabetes induction, the penis was removed for immunohistochemical staining of smooth muscle and neuronal nitric oxide synthase (nNOS) in midshaft penile tissues. The cross-sectional areas of the whole midshaft penis and the corpora cavernosa were quantified. The smooth muscle in the corpora cavernosa and nNOS in the dorsal nerves were quantified. The weight, but not the length, of the penis was lower in diabetics. The cross-sectional areas of the total midshaft penis and the corpora cavernosa were lower in diabetic rats compared with controls 9 weeks, but not 3 weeks after diabetes induction. The cross-sectional area of smooth muscle in the corpora cavernosa as percentage of the overall area of the corpora cavernosa was lower in diabetic rats than in controls 9 weeks, but not 3 weeks after diabetes induction. Percentage change of nNOS in dorsal nerves was similar at 3 weeks, and has a decreased trend at 9 weeks in diabetic rats compared with controls. Diabetes causes temporal alterations in the penis, and the significant changes in STZ rat model begin 3-9 weeks after induction. Further studies on the reversibility of the observed changes are warranted.