Amino acid derivative-mediated detoxification and functionalization of dual cure dental restorative material for dental pulp cell mineralization
Amino acid derivative-mediated detoxification and functionalization of dual cure dental restorative material for dental pulp cell mineralization
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DOI：
10.1016/j.biomaterials.2010.06.018
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发表时间：
2010-10-01
期刊：
影响因子：
14
通讯作者：
Ogawa, Takahiro
中科院分区：
文献类型：
--
作者：
Minamikawa, Hajime;Yamada, Masahiro;Ogawa, Takahiro
目前的牙科修复材料由于其细胞毒性，仅用于填补牙本质和牙釉质等硬组织的缺损。因此，深洞中暴露的牙髓组织必须首先被氢氧化钙等盖髓材料覆盖，以形成一层矿化组织。然而，这种组织矿化是基于病理反应，并引发持久的炎症，经常导致临床问题。本研究测试了N-乙酰半胱氨酸（NAC），氨基酸衍生物，以减少细胞毒性和诱导矿化组织的导电性树脂改性玻璃离子（RMGI），广泛使用的牙科修复材料具有双重固化机制的能力。将大鼠牙髓细胞培养在未经处理或添加NAC的RMGI上。NAC的补充大大增加了24小时孵育后的活细胞的百分比从46.7%至73.3%。细胞附着，扩展，增殖活性，成牙本质细胞相关的基因和蛋白表达显着增加NAC补充RMGI。细胞的矿化能力，这是几乎抑制未经处理的RMGI，诱导NAC补充RMGI。这些改善的行为和功能的牙髓细胞NAC补充RMGI与细胞内活性氧的产生相当大的减少和细胞内谷胱甘肽储备水平的增加。这些结果表明，NAC可以解毒和功能化RMGIs通过两种不同的机制，涉及原位材料解毒和抗氧化细胞保护。我们相信，这项研究提供了一种新的方法，为发展牙科修复材料，使矿化组织再生。(C)2010爱思唯尔有限公司版权所有。
Current dental restorative materials are only used to fill the defect of hard tissues, such as dentin and enamel, because of their cytotoxicity. Therefore, exposed dental pulp tissues in deep cavities must be first covered by a pulp capping material like calcium hydroxide to form a layer of mineralized tissue. However, this tissue mineralization is based on pathological reaction and triggers long-lasting inflammation, often causing clinical problems. This study tested the ability of N-acetyl cysteine (NAC), amino acid derivative, to reduce cytotoxicity and induce mineralized tissue conductivity in resin-modified glass ionomer (RMGI), a widely used dental restorative material having dual cure mechanism. Rat dental pulp cells were cultured on untreated or NAC-supplemented RMGI. NAC supplementation substantially increased the percentage of viable cells from 46.7 to 73.3% after 24-h incubation. Cell attachment, spreading, proliferative activity, and odontoblast-related gene and protein expressions increased significantly on NAC-supplemented RMGI. The mineralization capability of cells, which was nearly suppressed on untreated RMGI, was induced on NAC-supplemented RMGI. These improved behaviors and functions of dental pulp cells on NAC-supplemented RMGI were associated with a considerable reduction in the production of intracellular reactive oxygen species and with the increased level of intracellular glutathione reserves. These results demonstrated that NAC could detoxify and functionalize RMGIs via two different mechanisms involving in situ material detoxification and antioxidant cell protection. We believe that this study provides a new approach for developing dental restorative materials that enables mineralized tissue regeneration. (C) 2010 Elsevier Ltd. All rights reserved.