SV40 DNA: quantitative conversion of closed circular to open circular form by an ethidium bromide-restricted endonuclease.
SV40 DNA: quantitative conversion of closed circular to open circular form by an ethidium bromide-restricted endonuclease.
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SV40 DNA：通过溴化乙锭限制性核酸内切酶将闭合环状定量转化为开放环状。
DOI：
10.1016/0022-2836(73)90062-4
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发表时间：
1973
影响因子：
5.6
通讯作者：
R. Barzilai
中科院分区：
文献类型：
--
作者：
R. Barzilai
在溴化乙锭存在下，用核酸内切酶消化，可将SV40 DNA的闭环分子定量转化为开环形式。在这些条件下，几乎没有分子被消化到开环形式之外，而在没有染料的情况下，DNA被完全片段化。
Closed circular molecules of SV40 DNA can be quantitatively converted to the open circular form by digestion with endonucleases in the presence of ethidium bromide. Under these conditions almost no molecules were digested beyond the open circular form, whereas in the absence of the dye the DNA was completely fragmented.