Effects of sodium butyrate stimulation on immune-related mRNA-miRNA network in intestine of grass carp
Effects of sodium butyrate stimulation on immune-related mRNA-miRNA network in intestine of grass carp
复制标题
丁酸钠刺激对草鱼肠道免疫相关mRNA-miRNA网络的影响
DOI：
10.1111/are.14894
复制
发表时间：
2021
影响因子：
2
通讯作者：
Liu Zhen
中科院分区：
文献类型：
--
作者：
Tang Xiangbei;Tang Jianzhou;Zhou Yi;Qu Fufa;Zou Jun;Xiao Tiaoyi;Liu Zhen
丁酸钠是畜牧业中应用最广泛的饲料添加剂之一。近年来，丁酸钠作为补充剂在水产养殖中的应用日益增多。本研究旨在研究饲料中添加丁酸钠对中国重要养殖物种草鱼肠道m RNA和m RNA的影响。以Illumina HiSeq 2,500为平台，采用RNA-Seq技术，获得了饲喂0、1.0、2.5、5.0、7.5和10.0 mg/kg丁酸钠饲料的草鱼肠道组织的mRNA和microRNA图谱。饲养试验用18尾1龄草鱼(每组3尾)，在实验室水箱中饲养40天。从6个肠道文库中共获得349,860,852个序列读数。对差异表达基因的功能分析表明，丁酸钠补充后，参与免疫途径的基因往往被激活。共获得700个microRNAs，包括275个保守的microRNAs和425个可能参与调控14,300个基因的新的microRNAs。斯皮尔曼的相关性分析确定了18对与免疫途径相关的微RNA-−(P<；T.01和R<；T.05)。MicroRNAs靶向的潜在基因包括CXCL12、AKT1S1、Cab39和MHCII，它们是与肠道免疫途径相关的重要基因。据我们所知，这是第一次在添加丁酸钠的情况下对草鱼肠道中的mRNA和microRNA进行整合。本研究结果提示，丁酸钠可能通过调节鱼类的microRNA-mRNA相互作用而影响肠道免疫系统。
Sodium butyrate is one of the most popular feed additives in animal husbandry. In recent years, sodium butyrate has been increasingly used as supplement in aquaculture. The present study is to investigate the intestinal mRNA and microRNA response to diet with sodium butyrate in grass carp (Ctenopharyngodon idella), an important aquaculture species in China. mRNA and microRNA profiles of intestine of grass carp fed with diet contained 0, 1.0, 2.5, 5.0, 7.5 and 10.0 g/kg sodium butyrate were obtained by RNA‐seq using Illumina Hiseq 2,500 platform. The feeding trial was performed using 18 individuals of 1‐year‐old grass carp (n= 3 for each group) and lasted for 40 days in tanks in laboratory. A total of 349,860,852 sequence reads were generated from six intestinal libraries. Functional analysis of differentially expressed genes showed that genes participated in immune pathways tend to be activated by sodium butyrate supplementation. A total of 700 microRNAs were obtained, including 275 conserved microRNAs and 425 novel microRNAs which are potentially involved in regulating 14,300 genes. Spearman's correlation analysis identified 18 pairs of microRNA‐mRNA associated with immune pathways (p< .01 and R<−0.5). The potential genes targeted by microRNAs include CXCL12, AKT1S1, Cab39 and MHCII which are important genes associated with intestinal immune pathways. To our knowledge, this is the first integrated profiling of both mRNA and microRNA in intestine with supplementation of sodium butyrate in grass carp. The present results suggest that sodium butyrate affects intestinal immune system by regulating microRNA‐mRNA interaction in fish.