Initial Characterization of a Subpopulation of Inherent Oscillatory Mammalian Olfactory Receptor Neurons
Initial Characterization of a Subpopulation of Inherent Oscillatory Mammalian Olfactory Receptor Neurons
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固有振荡哺乳动物嗅觉受体神经元亚群的初步表征
DOI：
10.1093/chemse/bjz052
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发表时间：
2019
期刊：
影响因子：
3.5
通讯作者：
Ache, Barry W
中科院分区：
文献类型：
--
作者：
Ukhanov, Kirill;Bobkov, Yuriy V;Martens, Jeffrey R;Ache, Barry W
已发表的证据表明，甲壳类动物固有的节律活跃或“爆发”初级嗅觉受体神经元（bORN）具有以前未描述过的功能特性，即通过气味刺激产生节律性来编码嗅觉信息。为了确定这种基于 bORN 的编码是否是甲壳类动物以外的嗅觉的基本特征，我们在小鼠体内贴片钳了类似 bORN 的 ORN，表征了它们的动态特性，并表明它们与龙虾 bORN 的动态特性一致。然后，我们通过对 OMP-GCaMP6f 小鼠的嗅觉上皮进行成像来表征 bORN 样活性。接下来，我们通过在 OR/GFP 小鼠中修补 ORN 来证明节律活性不依赖于内源性 OR。最后，我们展示了在小鼠中表征的类 bORN ORN 的特性，并推广到了大鼠。我们的研究结果表明，编码气味时间应被视为嗅觉的基本特征，有可能用于引导甲壳类动物和哺乳动物等多种动物的气味羽流。
Published evidence suggests that inherent rhythmically active or “bursting” primary olfactory receptor neurons (bORNs) in crustaceans have the previously undescribed functional property of encoding olfactory information by having their rhythmicity entrained by the odor stimulus. In order to determine whether such bORN-based encoding is a fundamental feature of olfaction that extends beyond crustaceans, we patch-clamped bORN-like ORNs in mice, characterized their dynamic properties, and show they align with the dynamic properties of lobster bORNs. We then characterized bORN-like activity by imaging the olfactory epithelium of OMP-GCaMP6f mice. Next, we showed rhythmic activity is not dependent upon the endogenous OR by patching ORNs in OR/GFP mice. Lastly, we showed the properties of bORN-like ORNs characterized in mice generalize to rats. Our findings suggest encoding odor time should be viewed as a fundamental feature of olfaction with the potential to be used to navigate odor plumes in animals as diverse as crustaceans and mammals.