Nitric oxide ejects electrons from the binuclear centre of cytochrome c oxidase by reacting with oxidised copper: a general mechanism for the interaction of copper proteins with nitric oxide?
Nitric oxide ejects electrons from the binuclear centre of cytochrome c oxidase by reacting with oxidised copper: a general mechanism for the interaction of copper proteins with nitric oxide?
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DOI：
10.1016/s0014-5793(97)01009-0
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发表时间：
1997-09-08
期刊：
影响因子：
3.5
通讯作者：
Wilson, MT
中科院分区：
文献类型：
--
作者：
Cooper, CE;Torres, J;Wilson, MT
NO浓度的小幅增加可以通过在细胞色素C氧化酶的双核Hem A(3)/Cu-B氧还原位置反应来抑制线粒体的氧耗，在这里我们证明在正常周转条件下，NO最初与氧化的幼崽反应，而不是与Hem A(3)反应。我们认为，初始的Cu+/NO+络合物水合生成亚硝酸盐、质子和CuB+，后者从双核中心射出一个电子，并导致观察到的酶中其他电子转移中心(HEMA和CUA)的减少。这些反应可能对NO与其他铜蛋白的相互作用具有重要意义。(C)1997年欧洲生化学会联合会。
Small increases in NO concentration can inhibit mitochondrial oxygen consumption by reacting at the binuclear haem a(3)/Cu-B oxygen reduction site of cytochrome c oxidase, Here we demonstrate that under normal turnover conditions NO reacts initially with the oxidised CUB rather than the haem a(3). We propose that hydration of an initial Cu+/NO+ complex forms nitrite, a proton and CUB+; the latter ejects an electron from the binuclear centre and results in the observed (100 s(-1)) reduction of other electron transfer centres in the enzyme (haem a and CUA) These reactions may have implications for the interactions of NO with other copper proteins. (C) 1997 Federation of European Biochemical Societies.