High-resolution molecular localization by freeze-fracture replica labeling.
High-resolution molecular localization by freeze-fracture replica labeling.
复制标题
通过冷冻断裂复制标记进行高分辨率分子定位。
DOI：
10.1007/978-1-60761-783-9_16
复制
发表时间：
2010
影响因子：
--
通讯作者：
T. Fujimoto
中科院分区：
文献类型：
--
作者：
Akikazu Fujita;T. Fujimoto
冷冻断裂技术将冻结的脂质双层膜一分为二，并通过真空蒸发铂和碳来固定膜蛋白和脂类。在用十二烷基硫酸钠处理除去膜外物质后，对标本进行免疫金标记，这提供了膜分子的二维分布及其与各种分化结构的关系的信息。与快速冷冻相结合，冷冻-断裂技术比其他使用传统化学固定的方法更具优势，因为可以在膜的大范围内观察到中尺度上的脂肪和蛋白质的分布。
The freeze-fracture technique splits the frozen lipid bilayer membrane into two halves and immobilizes membrane proteins and lipids by the vacuum evaporation of platinum and carbon. After a treatment by SDS to remove extramembrane materials, the specimen is subjected to immunogold labeling, which gives information on the two-dimensional distribution of membrane molecules and their relationship to various differentiated structures. In combination with rapid freezing, the freeze-fracture technique has an advantage over other methods using conventional chemical fixation because the distribution of lipids as well as proteins can be observed at the mesoscale in a wide area of the membrane.
影响因子：
2.5
作者：
Hagiwara, A;Fukazawa, Y;Shigemoto, R
通讯作者：
Shigemoto, R