No evidence for a direct extracellular interaction between human Fc receptor-like 3 (MAIA) and the sperm ligand IZUMO1
No evidence for a direct extracellular interaction between human Fc receptor-like 3 (MAIA) and the sperm ligand IZUMO1
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DOI：
10.1126/sciadv.adk6352
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发表时间：
2024-02-21
期刊：
影响因子：
13.6
通讯作者：
Wright，Gavin J.
中科院分区：
文献类型：
--
作者：
Bianchi，Enrica;Jimenez-Movilla，Maria;Wright，Gavin J.
受精涉及精子和卵子的识别和融合，以形成以前未被识别的有机体。在哺乳动物中，精子和卵子上的表面分子起着核心作用，虽然黏附是由IZUMO1-Juno精子-卵子配体-受体对介导的，但负责膜融合的分子/S仍然是个谜。最近，MAIA/FCRL3被鉴定为哺乳动物卵子受体，它与IZUMO1和Juno结合，因此可能在配子识别和融合中起到桥梁作用。在这里，我们使用敏感的检测细胞外蛋白结合的方法来研究MAIA与IZUMO1和Juno之间的相互作用。尽管使用了具有明显生化活性的试剂，但我们没有发现MaIA/FCRL3与IZUMO1或Juno之间有任何直接结合。在基于细胞的膜融合实验中，我们也没有观察到MAIA/FCRL3的融合活性。我们的发现鼓励人们在进一步研究MaIA/FCRL3在受精过程中所起的作用时保持谨慎。
Fertilization involves the recognition and fusion of sperm and egg to form a previously unidentified organism. In mammals, surface molecules on the sperm and egg have central roles, and while adhesion is mediated by the IZUMO1-JUNO sperm-egg ligand-receptor pair, the molecule/s responsible for membrane fusion remain mysterious. Recently, MAIA/FCRL3 was identified as a mammalian egg receptor, which bound IZUMO1 and JUNO and might therefore have a bridging role in gamete recognition and fusion. Here, we use sensitive assays designed to detect extracellular protein binding to investigate the interactions between MAIA and both IZUMO1 and JUNO. Despite using reagents with demonstrable biochemical activity, we did not identify any direct binding between MAIA/FCRL3 and either IZUMO1 or JUNO. We also observed no fusogenic activity of MAIA/FCRL3 in a cell-based membrane fusion assay. Our findings encourage caution in further investigations on the role played by MAIA/FCRL3 in fertilization.