A novel role of long non-coding RNAs in response to X-ray irradiation
A novel role of long non-coding RNAs in response to X-ray irradiation
复制标题
长链非编码 RNA 在 X 射线照射响应中的新作用。
DOI：
10.1016/j.tiv.2015.09.007
复制
发表时间：
2015-12-25
影响因子：
3.2
通讯作者：
Jiao, Yang
中科院分区：
文献类型：
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作者：
Nie, Jihua;Peng, Chaojun;Jiao, Yang
在本研究中，lncRNA的作用，在辐射诱导的DNA损伤和氧化应激进行了探讨，以提高我们的理解后，辐射诱导的毒性激活的生物学途径。通过ROS产生和γ-H2 AX形成的剂量依赖性增加来确定X射线辐射对人支气管上皮细胞系（HBE）的毒性，并使用lncRNA特异性微阵列观察和量化lncRNA表达的变化。115 lncRNA表达在X射线照射后以剂量依赖性方式增加。生物信息学预测算法确定这些lncRNA显著影响p53信号传导途径，更具体地说，影响BRCA 1转录因子和邻近BRCA 1的编码基因。我们的研究结果突出了lncRNA通过p53途径对X射线诱导的DNA损伤起作用的先前未表征的作用，并表明lncRNA可能作为辐射毒性的新指标。(C)2015爱思唯尔有限公司版权所有。
In the present study, the role of lncRNAs in response to radiation-induced DNA damage and oxidative stress were explored to improve our understanding of the biological pathways activated upon radiation-induced toxicity. The toxicity of X-ray radiation on human bronchial epithelial cell lines (HBE) was determined through a dose-dependent increase in ROS production and gamma-H2AX formation and changes to lncRNA expression was observed and quantified using lncRNA-specific microarrays. 115 lncRNAs expression was increased in a dose-dependent manner following X-ray irradiation. Bioinformatic prediction algorithms determined that these lncRNAs significantly affect the p53 signaling pathway, and, more specifically, the BRCA 1 transcription factor and coding genes adjacent to BRCA 1. Our results highlight a previously uncharacterized role for lncRNAs to act via the p53-pathway in response to X-ray-induced DNA damage, and suggest lncRNAs may serve as novel indicators for radiation toxicity. (C) 2015 Elsevier Ltd. All rights reserved.