Retrograde labelling of dorsal root ganglion cells in the rat: A quantitative and morphological comparison of Fluoro-Gold with horseradish peroxidase labelling
Retrograde labelling of dorsal root ganglion cells in the rat: A quantitative and morphological comparison of Fluoro-Gold with horseradish peroxidase labelling
复制标题
大鼠背根神经节细胞的逆行标记：氟金与辣根过氧化物酶标记的定量和形态学比较
DOI：
10.1016/0304-3940(92)90332-2
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发表时间：
1992
影响因子：
2.5
通讯作者：
S. McMahon
中科院分区：
文献类型：
--
作者：
A. Baranowski;U. Anand;S. McMahon
我们已经比较了大鼠初级感觉神经元逆行标记荧光金（FG）和辣根过氧化物酶结合麦胚凝集素（HRP-WGA）。在48 h通过时间后的2.5%荧光金和在24和48 h后的5% FG逆行标记与HRP-WGA相似数量的细胞谱（P>0.1%学生t检验）。上述FG组的计算细胞大小分布与HRP-WGA相似。然而，24小时通过时间后，FG 2.5%标记的细胞显著较少（P<0.001学生t检验）。FG逆行转运可用于鉴定与HRP-WGA相同的DRG细胞群。
We have compared retrograde labelling of rat primary sensory neurons using Fluoro-Gold (FG) and horseradish peroxide conjugated with wheat germ agglutinin (HRP-WGA). Fluoro-Gold 2.5% after 48 h transit time and FG 5% after 24 and 48 h retrogradely labelled similar numbers of cell profiles as HRP-WGA (P>0.1% Student'st-test). The calculated cell size distribution for the above FG groups were similar to those for the HRP-WGA. However, FG 2.5% after a 24 h transit time labelled significantly fewer cells (P<0.001 Student'st-test). FG retrograde transport may be used to identify the same population of DRG cells as HRP-WGA.