Chemotaxis in neutrophil-like HL-60 cells.
Chemotaxis in neutrophil-like HL-60 cells.
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DOI：
10.1007/978-1-60761-198-1_11
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发表时间：
2009
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影响因子：
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通讯作者：
Weiner, Orion D
中科院分区：
文献类型：
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作者：
Millius, Arthur;Weiner, Orion D
细胞内蛋白质和信号的不对称定位在轴突导向、内皮细胞侵袭和免疫细胞迁移过程中指导运动。在这些过程中，细胞运动在被称为趋化性的过程中受到外部化学信号的引导。特别是，在先天免疫系统中白细胞的迁移已经在人中性粒细胞样细胞系（HL - 60）中得到了研究。在此，我们描述了HL - 60细胞的维持和转染，并解释了如何用两种标准的趋化性测定来分析它们的行为。最后，我们展示了如何固定和染色极化细胞的肌动蛋白细胞骨架以用于荧光显微镜成像。
Asymmetric localization of intracellular proteins and signals directs movement during axon guidance, endothelial cell invasion, and immune cell migration. In these processes, cell movement is guided by external chemical cues in a process known as chemotaxis. In particular, leukocyte migration in the innate immune system has been studied in the human neutrophil-like cell line (HL-60). Here, we describe the maintenance and transfection of HL-60 cells and explain how to analyze their behavior with two standard chemotactic assays. Finally, we demonstrate how to fix and stain the actin cytoskeleton of polarized cells for fluorescent microscopy imaging.