Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche
Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche
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DOI：
10.1038/nm1630
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发表时间：
2007-10-01
期刊：
影响因子：
82.9
通讯作者：
Young, Marian F.
中科院分区：
文献类型：
--
作者：
Bi, Yanming;Ehirchiou, Driss;Young, Marian F.
修复受损的肌腱仍然是一个巨大的挑战，这主要是因为缺乏对肌腱细胞及其前体的深入描述。我们发现人和小鼠的肌腱具有独特的细胞群，称为肌腱干细胞/祖细胞(TSPC)，具有普遍的干细胞特性，如克隆性、多能性和自我更新能力。分离的TSPC经体外扩增和体内移植后可再生肌腱样组织。此外，我们发现TSPC存在于一个主要由细胞外基质组成的独特的生态位中，我们发现Biglycan(BGN)和Fmod(Fmod)是组织这个生态位的两个关键成分。BGN和Fmod的缺失通过调节骨形态发生蛋白信号影响TSPC的分化，并损害体内肌腱的形成。我们的结果为肌腱细胞的生物学提供了新的见解，可能有助于未来治疗肌腱疾病的策略。
The repair of injured tendons remains a great challenge, largely owing to a lack of in- depth characterization of tendon cells and their precursors. We show that human and mouse tendons harbor a unique cell population, termed tendon stem/ progenitor cells ( TSPCs), that has universal stem cell characteristics such as clonogenicity, multipotency and self- renewal capacity. The isolated TSPCs could regenerate tendon- like tissues after extended expansion in vitro and transplantation in vivo. Moreover, we show that TSPCs reside within a unique niche predominantly comprised of an extracellular matrix, and we identify biglycan ( Bgn) and fibromodulin ( Fmod) as two critical components that organize this niche. Depletion of Bgn and Fmod affects the differentiation of TSPCs by modulating bone morphogenetic protein signaling and impairs tendon formation in vivo. Our results, while offering new insights into the biology of tendon cells, may assist in future strategies to treat tendon diseases.