Anti-adenovirus humoral responses influence on the efficacy of vaccines based on epitope display on adenovirus capsid
Anti-adenovirus humoral responses influence on the efficacy of vaccines based on epitope display on adenovirus capsid
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DOI：
10.1016/j.vaccine.2010.12.025
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发表时间：
2011-02-04
期刊：
影响因子：
5.5
通讯作者：
Benihoud, Karim
中科院分区：
文献类型：
--
作者：
Lanzi, Anastasia;Ben Youssef, Ghada;Benihoud, Karim
重组腺病毒(AdS)疫苗载体的有效性因阻碍基因转移的抗Ad抗体(Abs)的高度流行而降低。在Ad衣壳上展示抗原表位构成了一种绕过抗Ad抗体能力阻止抗原表达的另一种方法。为了了解表位插入位点的作用，我们将卵清蛋白衍生的表位基因插入到Ad Hexon或纤维蛋白中。六邻体修饰的ADS在一次注射后会引发更高的抗卵清蛋白抗体应答，但令人惊讶的是，纤维修饰的ADS在两次或几次注射后的效力要强得多。我们的数据揭示了抗Ad体液免疫在控制抗表位体液反应中的作用。(C)2010爱思唯尔有限公司。保留所有权利。
The efficacy of recombinant adenoviruses (Ads) vaccine vectors is diminished by the high prevalence of anti-Ad antibodies (Abs) that hampers gene transfer. Epitope display on Ad capsid constitutes an alternative approach to bypass anti-Ad Ab capacity from blocking antigen expression. To understand the role of the epitope insertion site, an ovalbumin-derived epitope was genetically inserted into either Ad hexon or fiber proteins. Hexon-modified Ads triggered higher anti-ovalbumin Ab responses after one injection but surprisingly fiber-modified Ads were by far more potent after two or several administrations. Our data unravel a role for anti-Ad humoral immunity in controlling anti-epitope humoral responses. (C) 2010 Elsevier Ltd. All rights reserved.