Relationship between cAMP production and protein secretion in rat submandibular acini: evidence for loss of reserve capacity with age.
Relationship between cAMP production and protein secretion in rat submandibular acini: evidence for loss of reserve capacity with age.
复制标题
大鼠颌下腺泡 cAMP 产生和蛋白质分泌之间的关系：随着年龄的增长储备能力丧失的证据。
DOI：
10.1016/0024-3205(94)00559-1
复制
发表时间：
1994
期刊：
影响因子：
6.1
通讯作者：
Scarpace,PJ
中科院分区：
文献类型：
--
作者：
Rajakumar,G;Scarpace,PJ
我们评估了异丙肾上腺素和福斯克林刺激幼年（4-7月龄）和老年（25月龄）雄性F-344大鼠下颌唾液腺cAMP生成和蛋白分泌。在衰老的腺泡中，最高剂量异丙肾上腺素（100 μM）的最大cAMP产量减少了3倍，蛋白质分泌减少了1 / 3。在异丙肾上腺素刺激下，年轻的腺泡比年老的腺泡对蛋白质分泌比cAMP产生更敏感。衰老大鼠cAMP生成和蛋白分泌的受体后刺激（福斯克林）减少。本报告证明了同时评估生化和功能相关因素的体外模型的有效性。此外，观察结果表明，随着年龄的增长，cAMP产生和蛋白质分泌的受体和受体后刺激都减少了。此外，年轻的大鼠在蛋白质分泌方面有一种cAMP生产的储备能力，这种储备能力在年老的大鼠中减少甚至没有。
We assessed isoproterenol and forskolin-stimulated cAMP production and protein secretion in submandibular salivary glands from young (4–7 mo) and old (25 mo) male F-344 rats. In senescent acini, the maximum amount of cAMP produced was three-fold less and the protein secreted was one-third less in response to the highest dose of isoproterenol (100 μM). Following isoproterenol stimulation, the young acini were more sensitive than the old for protein secretion than for cAMP production. Post-receptor stimulation (by forskolin) of cAMP production and protein secretion were less in the senescent rats. This report demonstrates the validity of anin vitromodel for simultaneous assessment of biochemical and functional correlates. Furthermore, the observations suggest that both receptor and post-receptor stimulation of cAMP production and protein secretion diminish with age. Moreover, there is a reserve capacity for cAMP production with respect to protein secretion in the young that is diminished or even absent in the old rats.