Analysis of codon:anticodon interactions within the ribosome provides new insights into codon reading and the genetic code structure
Analysis of codon:anticodon interactions within the ribosome provides new insights into codon reading and the genetic code structure
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DOI：
10.1017/s135583820100214x
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发表时间：
2001-07-01
期刊：
影响因子：
4.5
通讯作者：
Curran, JF
中科院分区：
文献类型：
--
作者：
Lim, VI;Curran, JF
虽然密码子：反密码子双链体的解码规则已基本阐明，但密码子：反密码子双链体“正确性”的物理化学原因一直不清楚。在这项工作中，在现有数据的基础上，我们提出正确的密码子：反密码子双链体是那些形成和相互作用的核糖体解码中心不伴随着氢和离子键的无偿损失。其他因素，如校对，碱基堆积和氨酰-tRNA浓度有助于氨酰-tRNA选择的效率和准确性，当然这些因素是重要的，但我们建议，单独的氢和离子键的分析提供了一个强大的一阶近似的解码精度。因此，我们的模型可以简化解码精度和错误的预测。该模型可以用数据进行改进，但已经足够强大，可以解释所有关于解码准确性的可用数据。在这里，我们预测哪些双链体应该被认为是正确的，哪些双链体负责几乎所有的误读，我们提出了一个进化方案，产生了遗传密码的混合盒。
Although the decoding rules have been largely elucidated, the physical-chemical reasons for the "correctness" of codon:anticodon duplexes have never been clear. In this work, on the basis of the available data, we propose that the correct codon:anticodon duplexes are those whose formation and interaction with the ribosomal decoding center are not accompanied by uncompensated losses of hydrogen and ionic bonds. Other factors such as proofreading, base-base stacking and aminoacyl-tRNA concentration contribute to the efficiency and accuracy of aminoacyl-tRNA selection, and certainly these factors are important; but we suggest that analyses of hydrogen and ionic bonding alone provides a robust first-order approximation of decoding accuracy. Thus our model can simplify predictions about decoding accuracy and error. The model can be refined with data, but is already powerful enough to explain all of the available data on decoding accuracy. Here we predict which duplexes should be considered correct, which duplexes are responsible for virtually all misreading, and we suggest an evolutionary scheme that gave rise to the mixed boxes of the genetic code.