Photosynthesis and sucrose metabolism in leaves of Arabidopsis thaliana aos, ein4 and rcd1 mutants as affected by wounding
Photosynthesis and sucrose metabolism in leaves of Arabidopsis thaliana aos, ein4 and rcd1 mutants as affected by wounding
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DOI：
10.1007/s11738-016-2309-1
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发表时间：
2017-01-01
影响因子：
2.6
通讯作者：
Ciereszko, Iwona
中科院分区：
文献类型：
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作者：
Lukaszuk, Edyta;Rys, Magdalena;Ciereszko, Iwona
我们已经调查了机械伤害拟南芥莲座叶光系统II的光化学活性，气体交换，糖含量和蔗糖代谢在野生型植物和突变体激素平衡受损的影响。已经使用了aos（茉莉酸缺乏）、rcd 1（对阿坝、乙烯和茉莉酸甲酯的敏感性降低）和ein 4（乙烯不敏感性）突变体。一般来说，机械损伤导致代谢的动态变化，特别是糖和类胡萝卜素的含量。与野生型植物相比，所有突变体都表现出较低的光合作用和呼吸作用，叶片损伤导致aos和ein4的呼吸作用降低，而野生型的呼吸作用增加。机械损伤引发蔗糖水解酶，如蔗糖合成酶（SuSy）和几种类型的转化酶的活性增加，这是最明显的情况下，rcd 1和aos植物。这是与损伤相关的变化，在突变体中的可溶性糖，但不是在野生型植物中的糖含量没有显着影响受伤。结果证实了应激激素，如茉莉酸和乙烯，在介导创伤后的应激反应的关键作用。实验结果还强调了SuSy和转化酶在受损组织再生中的重要作用，最有可能是通过提供细胞壁生物合成的前体和通过调节植物细胞中的糖信号传导。
We have investigated the influence of mechanical wounding of Arabidopsis rosette leaves on photochemical activity of photosystem II, gas exchange, sugar content and sucrose metabolism in wild-type plants and mutants impaired in hormonal balance. The aos (jasmonate deficiency), rcd1 (reduced sensitivity to ABA, ethylene, and methyl-jasmonate), and ein4 (ethylene insensitivity) mutants have been used. Generally, mechanical injury led to dynamic changes in metabolism, especially in sugar and carotenoid contents. Whereas all mutants showed lower photosynthesis and respiration in comparison to the wildtype plants, leaf wounding caused a decrease in respiration in aos and ein4, and an increase in respiration in wild type. The mechanical injury triggered an increase of the activities of sucrose hydrolysing enzymes, such as sucrose synthase (SuSy) and several types of invertases, which was most evident in case of rcd1 and aos plants. This was correlated with injury-related changes in soluble sugars in the mutants, but not in wild-type plants where sugar content was not significantly affected by wounding. The results confirm the key role of stress hormones, such as jasmonate and ethylene, in mediating stress responses after wounding. The outcome of the experiments also underlines important roles of SuSy and invertase in regeneration of injured tissues, most probably by providing precursors for cell wall biosynthesis and by modulating sugar-signalling in plant cells.