Activity of class III peroxidases in the defense of cotton to bacterial blight
Activity of class III peroxidases in the defense of cotton to bacterial blight
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DOI：
10.1094/mpmi.2003.16.11.1030
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发表时间：
2003-11-01
影响因子：
3.5
通讯作者：
Nicole, M
中科院分区：
文献类型：
--
作者：
Delannoy, E;Jalloul, A;Nicole, M
用野油菜黄单胞菌致病变种18号无毒小种侵染棉花品种Reba B50，使其子叶产生过敏反应。锦葵两组过氧化物酶与HR时程相关。早期，但短暂的积累过氧化物酶的材料封装在细胞间区域的细菌，观察在感染后3小时的免疫细胞化学，正好与氧化爆发。总愈创木酚过氧化物酶活性在经历HR的细胞中高度增加，从治疗后12小时。7个克隆的过氧化物酶基因的分子特征显示高度保守的B，D和F结构域，与植物III类过氧化物酶的相似性。基因表达的分析表明，在这两个兼容的（种族20）和不兼容的相互作用，这些基因：pod2，pod3，pod4和pod6的转录积累的变化。POD 4和POD 6在抗性期间比在疾病期间和对照中更强烈地上调，而POD 3在氧化爆发后的HR期间特异性下调。POD 2受病原菌感染诱导，在对照中受到弱刺激。这些数据表明，棉花过氧化物酶可能在防御黄单胞菌感染的反应中具有多种功能。
Cotton cotyledons displayed a hypersensitive reaction (HR) in the cultivar Reba B50 after infiltration with the avirulent race 18 from Xanthomonas campestris pv. malvacearum. Two sets of peroxidases were associated with the HR time course. Early but transient accumulation of peroxidase in material encapsulating the bacteria in intercellular areas was observed by immunocytochemistry at 3 h postinfection and coincided with the oxidative burst. Total guaiacol-peroxidase activity was highly increased in cells undergoing HR, from 12 h after treatment. Molecular characterization of seven cloned peroxidase genes revealed highly conserved B, D, and F domains, with similarities to plant class III peroxidases. Analysis of gene expression showed variation in transcript accumulation during both compatible (race 20) and incompatible interactions for four of these genes: pod2, pod3, pod4, and pod6. Pod4 and pod6 were more intensely up-regulated during resistance than during disease and in the control, while pod3 was specifically down-regulated during the HR after the oxidative burst. Pod2 was induced by pathogen infection and weakly stimulated in the control. These data suggest that cotton peroxidases may have various functions in the defense response to Xanthomonas infections.