Pathogenicity of some isolates of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorokin on 2nd and 4th larval instars of Colorado potato beetle, Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), under laboratory conditions
Pathogenicity of some isolates of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorokin on 2nd and 4th larval instars of Colorado potato beetle, Leptinotarsa decemlineata (Say) (Col.: Chrysomelidae), under laboratory conditions
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DOI：
10.5897/ajmr12.1112
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发表时间：
2012-09-06
影响因子：
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通讯作者：
Safavi, Seyed Ali
中科院分区：
文献类型：
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作者：
Akbarian, Jamshid;Ghosta, Youbert;Safavi, Seyed Ali
科罗拉多州马铃薯甲虫Leptinotarsa decemlineata(Say)是马铃薯的主要经济害虫之一。研究了球孢白僵菌Bals.6个菌株的致病性。维尔。(4个土生菌和2个非土生菌)和2个土生金龟子绿僵菌(Metsch.)都被调查过。用5种不同浓度的1×10(7)、5×10(7)、1×10(8)、5×10(8)和1×10(9)分生孢子ml(-1)对2龄和4龄幼虫进行生物测定。结果表明，最高浓度的球孢白僵菌对2龄和4龄幼虫的致死率分别为78.88%和44.44%。此外，AKB和其他治疗方法之间的死亡率也有显着差异。其他菌株LRC107、IRAN429C、LRC137、IRAN441C和Z-1分别在随后的班级中得分。两个绿僵菌菌株DEMI001和IRAN437C对幼虫的死亡率均低于球孢杆菌菌株。在所有实验中，随着分生孢子浓度的增加，死亡率都有所增加。根据我们的结果，2龄幼虫比4龄幼虫更敏感。
The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is one of the major economically pests of potato throughout the world. In this study, the pathogenicity of six isolates of Beauveria bassiana (Bals.) Vuill. (four indigenous and two non indigenous isolates) and two indigenous isolates of Metarhizium anisopliae (Metsch.) were investigated. Five different concentrations from each isolate, 1x10(7), 5x10(7), 1x10(8), 5x10(8) and 1x10(9) conidia ml(-1), were applied in bioassays on 2nd and 4th instars larvae. Results showed that the percentage of mortality due to AKB isolate of B. bassiana at the highest concentration were 78.88 and 44.44, for 2nd and 4th instars larvae, respectively. Also there were significant differences in percentage of mortality between AKB and other treatments. Other isolates, LRC107, IRAN429C, LRC137, IRAN441C and Z-1 were scored in the subsequent classes, respectively. Both M. anisopliae isolates, DEMI001 and IRAN437C showed the lowest mortality rate on larvae in comparison with B. bassiana isolates. In all experiments, with increase in conidial concentration, the mortality rate was increased. Based on our results, the 2nd instar larvae were more susceptible than the 4th instar.