Prevalence and characterization of Theileria and Babesia species in cattle under different husbandry systems in western Uganda.
Prevalence and characterization of Theileria and Babesia species in cattle under different husbandry systems in western Uganda.
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乌干达西部不同畜牧系统下牛中泰勒虫和巴贝虫的流行率和特征。
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
C. Waiswa
中科院分区：
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作者：
D. Muhanguzi;E. Matovu;C. Waiswa
使用反向线印迹杂交测定 (RLB) 对来自卡沙里县 6 个县的总共 363 头牛进行了泰勒虫属和巴贝虫属物种的检测。泰勒虫属和巴贝虫属的患病率分别为 1·9.8%（CI = 9·5%、15.7-23.9%）和 0·.6%（CI = 9·5%、-0.2-1.4%），其中至少 68%（CI = 95%、63.2-72.8）双重感染超过一种泰勒虫属。泰勒虫属检测到的包括； T. parva、T. mutans、T. taurotragi、T. vilifera、T. buffeli、T. spp。 （黑貂），T. spp。 （水牛）和 T. bicornis 为 24% (CI = 95%, 19.6-28.4%)、18.4% (CI = 95%, 14.4-22.4%)、14% (CI = 95%, 10.4-17.6%)、13.7% (CI = 95%, 10.2-17.2%)、分别为 12.6%（CI = 95%、9.2-16.0%）、10.4%（CI = 95%、7.26-13.54%）、4.4%（CI = 95%、2.3-6.5%）和 3.8%（CI = 95%、1.8-5.8%）。介绍并讨论了不同牛年龄组、品种、管理系统和原产地亚县中不同泰勒虫属和巴贝虫属的流行情况。在逻辑回归中观察到，与本地和外来品种相比，杂交牛的感染风险是本地和外来品种的 2.5 倍（OR = 2.5，95% CI；1.44-4.49）。无论牛的品种类型如何；杀螨剂的施用率、限制犊牛活动、限制放牧（围场）和零放牧是决定结核病感染风险的最重要参数。 RLB 检测到动物感染，泰勒虫属和巴贝虫属特异性 PCR 呈阴性。这些动物的寄生虫血症较低，无法通过此类非物种特异性 PCR 检测到。因此，RLB 是一种非常敏感和特异的诊断工具，应在乌干达蜱传血寄生虫流行病学研究中采用。
A total of 363 cattle taken from six sub counties of Kashaari county were tested for presence of Theileria and Babesia species using reverse line blot hybridization assay (RLB). The prevalences of Theileria and Babesia species were found to b e 1 9.8% ( CI = 9 5%, 15.7-23.9%) and 0 .6% ( CI = 9 5%, -0.2-1.4%) respectively with at least 68% (CI = 95%, 63.2-72.8) dually infected with more than one Theileria sp. Theileria sp. detected include; T. parva, T. mutans, T. taurotragi, T. vilifera, T. buffeli, T. spp. (sable), T. spp. (buffalo) and T. bicornis at 24% (CI = 95%, 19.6-28.4%), 18.4% (CI = 95%, 14.4-22.4%), 14% (CI = 95%, 10.4-17.6%), 13.7% (CI = 95%, 10.2-17.2%), 12.6% (CI = 95%, 9.2-16.0%), 10.4% (CI = 95%, 7.26-13.54%), 4.4% (CI = 95%, 2.3-6.5%) and 3.8% (CI = 95%, 1.8-5.8%) respectively. The prevalences of different Theileria and Babesia species among different cattle age groups, breeds, management systems and sub county of origin are presented and discussed. A 2.5 times risk of infection associated with cross bred cattle (OR = 2.5, 95% CI; 1.44-4.49) compared to that of local and exotic breeds was observed on logistic regression. Regardless of type of cattle breed; rate of acaracide application, restriction of calf movement, restricted grazing (paddocking) and zero gr azing wer e t he mos t i mportant pa rameters t hat determined the r isk of infection with TBs. RLB detected infections in animals wh ich were negative by Theileria and Babesia Genera specific PCR. Such animals had low parasitemia that could not be detected by such non species-specific PCR. RLB is therefore a very sensitive and specific diagnostic tool that should be adopted in tick-borne hemoparasite epidemiological studies in Uganda.