Effect of a collagen matrix containing epidermal growth factor on wound contraction
Effect of a collagen matrix containing epidermal growth factor on wound contraction
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DOI：
10.1046/j.1524-475x.1998.60307.x
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发表时间：
1998-05-01
影响因子：
2.9
通讯作者：
Shioya, Nobuyuki
中科院分区：
文献类型：
--
作者：
Inoue, Masayuki;Ono, Ichiro;Shioya, Nobuyuki
已知伤口过度收缩会导致病理性伤口挛缩。使用兔子模型，我们将牛 I 型胶原基质海绵作为真皮替代品和人表皮生长因子应用于全层切除伤口。受伤后14天和28天评估伤口收缩情况。结果发现，在受伤28天后，单独用胶原基质治疗或用0.1和1杯表皮生长因子治疗的所有伤口中，胶原基质和表皮生长因子均显着抑制伤口收缩（p < 0.001）。有趣的是，与单独使用基质相比，胶原基质与表皮生长因子的组合强烈抑制伤口收缩（第28天p < 0.01）。组织学分析显示，在用这些物质处理的伤口下，但在未经处理的伤口下，真皮中的胶原纤维没有规则的水平排列。此外，使用成纤维细胞填充的胶原凝胶，还观察到表皮生长因子对成纤维细胞凝胶收缩的直接抑制作用。培养6天后测定，未经刺激的胶原凝胶收缩至其原始尺寸的29.5±0.6%。 3天或更长时间，表皮生长因子抑制成纤维细胞的胶原凝胶收缩（6天后：在1、10和100ng/ml表皮生长因子下，34.2±1.8%，p>0.05；36.5±2.8%，p<0.05；和39.8±2.1%，p<0.001，分别）。总之，胶原蛋白基质和表皮生长因子，特别是组合使用，可能有助于预防伤口挛缩。
Excessive wound contraction is known to lead to pathological wound contracture. Using a rabbit model, we applied a bovine type I collagen matrix sponge as a dermal substitute and human epidermal growth factor to full-thickness excisional wounds. Wound contraction was assessed 14 and 28 days after wounding. It was found that both collagen matrix and epidermal growth factor significantly inhibited wound contraction (p < 0.001) in all wounds treated with collagen matrix alone or treated with 0.1 and 1 mug of epidermal growth factor 28 days after wounding. Interestingly, the combination of collagen matrix with epidermal growth factor strongly inhibited wound contraction over matrix alone (p < 0.01 on day 28). Histological analyses showed a regular horizontal arrangement of collagen fibers in the dermis under wounds treated with these substances but not under untreated wounds. Furthermore, using a fibroblast-populated collagen gel, the direct inhibitory effect of epidermal growth factor on gel contraction by fibroblasts was also observed. Collagen gels without stimulation contracted to 29.5 +- 0.6% of their original size, as determined 6 days after culturing. At 3 days or more, epidermal growth factor inhibited collagen gel contraction by fibroblasts (after 6 days: 34.2 +- 1.8%, p > 0.05; 36.5 +- 2.8%, p < 0.05: and 39.8 +- 2.1%, p < 0.001 at 1, 10, and 100 ng/ml of epidermal growth factor, respectively). In conclusion, collagen matrix and epidermal growth factor, particularly in combination, may be useful in the prevention of wound contracture.