Facile synthesis of near infrared fluorescent trypsin-stabilized Ag nanoclusters with tunable emission for 1,4-dihydronicotinamide adenine dinucleotide and ethanol sensing.
Facile synthesis of near infrared fluorescent trypsin-stabilized Ag nanoclusters with tunable emission for 1,4-dihydronicotinamide adenine dinucleotide and ethanol sensing.
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DOI：
10.1016/j.aca.2015.07.001
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发表时间：
2015-07
影响因子：
6.2
通讯作者：
Siyu Liu;Hui Wang;Zhen Cheng;Hongguang Liu
中科院分区：
文献类型：
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作者：
Siyu Liu;Hui Wang;Zhen Cheng;Hongguang Liu
采用一种简便的化学合成方法制备了近红外荧光胰蛋白酶稳定的银纳米团簇（Try-Ag NC）。通过简单调节合成体系的pH值，可以调节所制备的Try-Ag纳米粒子的荧光发射波长，并且Try-Ag纳米粒子具有对称的荧光激发和发射峰。Try-Ag NCs的荧光在各种离子和分子存在下保持恒定，并且它可以被1，4-二氢烟酰胺腺嘌呤二核苷酸（NADH）而不是其氧化形式的烟酰胺腺嘌呤二核苷酸（NAD+）有效地猝灭。这一特性使Try-Ag NCs成为一种新的分析平台，用于监测与NADH有关的生物反应。本研究还利用乙醇脱氢酶催化NAD+与乙醇反应生成NADH的特性，将Try-Ag纳米粒子应用于乙醇的分析。该平台可在10 ~ 300 μmol/L范围内测定乙醇，检出限为5 μmol/L。
A facile chemical synthetic route was developed to prepare near-infrared fluorescent trypsin-stabilized Ag nanoclusters (Try-Ag NCs). The fluorescence emission wavelength of the produced Try-Ag NCs is tunable by simple adjusting pH value of the synthesis system, and the Try-Ag NCs offer a symmetric fluorescent excitation and emission peak. The fluorescence of Try-Ag NCs remains constant in the presence of various ions and molecules, and it can be effectively quenched by 1,4-dihydronicotinamide adenine dinucleotide (NADH) instead of its oxidized forms nicotinamide adenine dinucleotide (NAD+). This property enables the Try-Ag NCs to be a novel analytical platform to monitor biological reaction involved with NADH. In this work, the Try-Ag NCs was also applied to analyze ethanol based on the generation of NADH which was the product of NAD+and ethanol in the catalysis of alcohol dehydrogenase. And the proposed platform allowed ethanol to be determined in the range from 10 to 300 μmol/L with 5 μmol/L detection limit.