Gastrointestinal Simulation Model TWIN-SHIME Shows Differences between Human Urolithin-Metabotypes in Gut Microbiota Composition, Pomegranate Polyphenol Metabolism, and Transport along the Intestinal Tract
Gastrointestinal Simulation Model TWIN-SHIME Shows Differences between Human Urolithin-Metabotypes in Gut Microbiota Composition, Pomegranate Polyphenol Metabolism, and Transport along the Intestinal Tract
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DOI：
10.1021/acs.jafc.7b02049
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发表时间：
2017-07-12
影响因子：
6.1
通讯作者：
Tomas-Barberan, Francisco A.
中科院分区：
文献类型：
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作者：
Garcia-Villalba, Rocio;Vissenaekens, Hanne;Tomas-Barberan, Francisco A.
采用TWIN-SHINE系统比较了两个不同尿素代谢型个体的肠道微生物群对石榴多酚的代谢。肠道菌群、鞣花单宁代谢、短链脂肪酸（SCFA）、代谢物运输和Caco-2细胞的II期代谢进行了探讨。模拟再现了每种代谢型的体内代谢谱。该研究首次表明，微生物组成、鞣花单宁的代谢和短链脂肪酸在不同代谢类型和大肠中存在差异。实验还表明，石榴酚类物质可以保持肠道细胞的完整性。石榴多酚提高尿素和丙酸的产生，以及阿克曼氏菌和戈登杆菌的患病率，其中降结肠的影响最大。该系统提供了对石榴多酚肠道微生物群代谢和肠道细胞吸收机制的深入了解。SHIME/Caco-2联合细胞系统获得的结果与先前的人类和动物研究一致，并表明尽管尿素代谢物由于肠肝循环而沿胃肠道存在，但它们主要在远端结肠区域产生。
A TWIN-SHINE system was used to compare the metabolism of pomegranate polyphenols by the gut microbiota from two individuals with different urolithin metabotypes. Gut microbiota, ellagitannin metabolism, short-chain fatty acids (SCFA), transport of metabolites, and phase II metabolism using Caco-2 cells were explored. The simulation reproduced the in vivo metabolic profiles for each metabotype. The study shows for the first time that microbial composition, metabolism of ellagitannins, and SCFA differ between metabotypes and along the large intestine. The assay also showed that pomegranate phenolics preserved intestinal cell integrity. Pomegranate polyphenols enhanced urolithin and propionate production, as well as Akkermansia and Gordonibacter prevalence with the highest effect in the descending colon. The system provides an insight into the mechanisms of pomegranate polyphenol gut microbiota metabolism and absorption through intestinal cells. The results obtained by the combined SHIME/Caco-2 cell system are consistent with previous human and animal studies and show that although urolithin metabolites are present along the gastrointestinal tract due to enterohepatic circulation, they are predominantly produced in the distal colon region.