A diallel analysis of heterosis in elite hybrid rice based on RFLPs and microsatellites
A diallel analysis of heterosis in elite hybrid rice based on RFLPs and microsatellites
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DOI：
10.1007/bf00225139
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发表时间：
1994-10
影响因子：
5.4
通讯作者：
Qifa Zhang;Y. J. Gao;S. Yang;R. Ragab;M. Maroof;Zhaohong Li
中科院分区：
文献类型：
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作者：
Qifa Zhang;Y. J. Gao;S. Yang;R. Ragab;M. Maroof;Zhaohong Li
杂交水稻为世界水稻产量的大幅增长做出了巨大贡献。尽管如此，很少有人注意研究水稻杂种优势的遗传基础。本文报道了利用限制性片段长度多态性（RFLP）和微卫星两类分子标记进行杂种优势的分析。8行，这代表了一个显着的部分杂交水稻种质，在所有可能的对，F1代进行了评价，产量和产量构成性状在一个重复的田间试验。利用117个RFLP探针和10个微卫星标记对亲本进行多态性分析，共获得76个多态性标记。结果表明，这些杂交组合普遍存在高水平的杂种优势，许多F1代的中亲优势和双亲优势均在100%以上，包括一些保持系间的杂交。产量性状的杂种优势远高于产量构成性状，几乎完全符合乘法模型。16 ~ 30个标记位点（阳性标记）对产量或产量构成性状有极显著影响。杂合性与几个性能和杂种优势的属性显着相关。基于正标记（特定杂合度）的相关性较大的中亲优势的产量和种子/穗，也为F1粒重。这些较大的相关性对预测杂种优势可能具有实用价值。
Hybrid rice has contributed significantly to the dramatic increase of rice production in the world. Despite this, little attention has been given to studying the genetic basis of heterosis in rice. In this paper, we report a diallel analysis of heterosis using two classes of molecular markers: restriction fragment length polymorphisms, (RFLPs) and microsatellites. Eight lines, which represent a significant portion of hybrid rice germ plasm, were crossed in all possible pairs, and the F1s were evaluated for yield and yield component traits in a replicated field trial. The parental lines were surveyed for polymorphisms with 117 RFLP probes and ten microsatellites, resulting in a total of 76 polymorphic markers well-spaced in the rice RFLP map. The results indicated that high level heterosis is common among these crosses: more than 100% midparent and 40% better-parent heterosis were observed in many F1s, including some crosses between maintainer lines. Heterosis was found to be much higher for yield than for yield component traits, which fits a multiplicative model almost perfectly. Between 16 and 30 marker loci (positive markers) detected highly significant effects on yield or its component traits. Heterozygosity was significantly correlated with several attributes of performance and heterosis. Correlations based on positive markers (specific heterozygosity) were large for midparent heterosis of yield and seeds/panicle and also for F1kernel weight. These large correlations may have practical utility for predicting heterosis.