Immune-Enhancing Effect of Fermented Maesil (Prunus mume Siebold & Zucc.) with Probiotics against Bordetella bronchiseptica in Mice
Immune-Enhancing Effect of Fermented Maesil (Prunus mume Siebold & Zucc.) with Probiotics against Bordetella bronchiseptica in Mice
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DOI：
10.1292/jvms.09-0555
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发表时间：
2010-09-01
影响因子：
1.2
通讯作者：
Lee, Bong-Joo
中科院分区：
文献类型：
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作者：
Jung, Bock-Gie;Ko, Jae-Hyung;Lee, Bong-Joo
Maesil（梅花）在东亚国家长期以来被用作传统药物和健康食品。它具有许多有益的生物活性，包括针对病原体的潜在抗菌作用。益生菌还具有抗菌作用。此外，一些益生菌在调节免疫系统方面具有重要作用。本研究评估了发酵的 Msesil 与益生菌（酿酒酵母、枯草芽孢杆菌和嗜酸乳杆菌）对小鼠的免疫增强作用，特别是对支气管败血博德特氏菌的作用，作为开发饲料补充剂以促进免疫活性和预防疾病（尤其是猪）的第一步。持续摄入含有益生菌的发酵Masesil可显着增加外周血中巨噬细胞比例和脾脏中T淋巴细胞比例。此外，与对照组相比，喂食发酵 Maesil 的小鼠中针对福尔马林杀死的支气管败血杆菌的抗体产量显着增加。在实验性支气管败血症感染后第 3 天（最大峰值时间），与对照组相比，从发酵的 Maesil 喂养的动物获得的支气管肺泡灌洗液中的白细胞数量显着更高。此外，感染后7天，与对照组相比，饲喂发酵Maesil的小鼠脾细胞中肿瘤坏死因子-α和干扰素-γ的相对信使RNA表达水平显着增加。总而言之，这些研究结果表明，含有发酵 Maesil 和益生菌的饲料可通过有效刺激非特异性免疫反应，增强小鼠的免疫活性，尤其是针对支气管败血性博德氏菌的免疫活性。
Maesil (Prunus mume) has long been used as a traditional drug and healthy food in East Asian countries. It possesses a number of beneficial biological activities including potential antimicrobial effects against pathogens. Probiotics also have antibacterial effects. Moreover, some probiotics have an important role in regulating the immune system. The present study evaluated the immune enhancing effects of fermented Msesil with probiotics (Saccharomyces cerevisiae, Bacillus subtilis and Lactobacillus acidophilus) in mice, especially against Bordetella bronchiseptica, as an initial step towards the development of feed supplements for the promotion of immune activity and prevention of disease, especially in pigs. Continuous ingestion of fermented Masesil with probiotics markedly increased the macrophage ratio in peripheral blood and the T lymphocyte ratio in the spleen. In addition, antibody production against formalin-killed B. bronchiseptica significantly increased in the mice fed fermented Maesil compared with the control group. The number of leukocytes was significantly higher in the bronchio-alveolar lavage obtained from the fermented Maesil-fed animals compared to it in the control group at day 3 (maximal peak time) after experimental B. bronchiseptica infection. Moreover, at 7 day post-infection, relative messenger RNA expression levels of tumor necrosis factor-alpha and interferon-gamma were significantly increased in splenocytes of mice fed fermented Maesil compared with those in the control group. Taken together, these findings suggest that feed containing fermented Maesil with probiotics enhances immune activity in mice, especially against B. bronchiseptica, via the potent stimulation of non-specific immune responses.