Prevalence of antimicrobial resistance in relation to virulence genes and phylogenetic origins among urogenital Escherichia coli isolates from dogs and cats in Japan
Prevalence of antimicrobial resistance in relation to virulence genes and phylogenetic origins among urogenital Escherichia coli isolates from dogs and cats in Japan
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DOI：
10.2460/ajvr.73.3.409
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发表时间：
2012-03-01
影响因子：
1
通讯作者：
Takahashi, Toshio
中科院分区：
文献类型：
--
作者：
Harada, Kazuki;Niina, Ayaka;Takahashi, Toshio
评估抗菌药物耐药性（AMR）的状况，确定肠外毒力因子（VF）和系统发育起源，并分析来自伴侣动物的肠外致病性大肠杆菌（ExPEC）分离株中这些性状之间的关系。从2003年至2010年期间收集的85只狗和19只猫的尿液或生殖器拭子样本中分离出104株大肠杆菌，程序-通过使用琼脂稀释法确定分离株的抗菌药物敏感性;多重PCR测定用于VF基因检测和系统发育组评估。通过随机扩增多态性DNA分析评估遗传多样性。结果104株分离株中，45株（43.3%）耐2种以上抗菌药物。B2、D、B1和A类群分别占64（61.5%）、22（21.2%）、13（12.5%）和5（4.8%）株。B2群菌株对所有抗菌药物的耐药性均较低，pap、hly和cnf基因的携带频率较高，aer基因的携带频率较低。aer基因与AMR直接相关，pap、sfa、hly和cnf基因与AMR呈负相关。随机扩增多态性DNA分析显示3个主要集群，主要由B1，B2和D组分离株; 2个亚群的B2组分离株有不同的VF和AMR status.Conclusions和临床相关性的流行率的多药耐药和人类的亲缘关系起源之间的ExPEC分离株在日本的伴侣动物是高的。这表明VF、系统发育起源和遗传多样性与ExPEC中的AMR显着相关。(Am J Vet Res 2012;73：409-417）
Objective-To assess the status of antimicrobial resistance (AMR), identify extraintestinal virulence factors (VFs) and phylogenetic origins, and analyze relationships among these traits in extraintestinal pathogenic Escherichia coli (ExPEC) isolates from companion animals.Sample-104 E coli isolates obtained from urine or genital swab samples collected between 2003 and 2010 from 85 dogs and 19 cats with urogenital infections in Japan.Procedures-Antimicrobial susceptibility of isolates was determined by use of the agar dilution method; a multiplex PCR assay was used for VF gene detection and phylogenetic group assessment. Genetic diversity was evaluated via randomly amplified polymorphic DNA analysis.Results-Of the 104 isolates, 45 (43.3%) were resistant to > 2 antimicrobials. Phylogenetically, 64 (61.5%), 22 (21.2%), 13 (12.5%), and 5 (4.8%) isolates belonged to groups B2, D, B1, and A, respectively. Compared with other groups, group B2 isolates were less resistant to all tested antimicrobials and carried the pap, hly, and cnf genes with higher frequency and the aer gene with lower frequency. The aer gene was directly associated and the pap, sfa, hly, and cnf genes were inversely associated with AMR. Randomly amplified polymorphic DNA analysis revealed 3 major clusters, comprised mainly of group B1, B2, and D isolates; 2 subclusters of group B2 isolates had different VF and AMR status.Conclusions and Clinical Relevance-Prevalences of multidrug resistance and human-like phylogenetic origins among ExPEC isolates from companion animals in Japan were high. It is suggested that VFs, phylogenetic origins, and genetic diversity are significantly associated with AMR in ExPEC. (Am J Vet Res 2012;73:409-417)