Preparation, characterization, and antioxidant properties of gallic acid-grafted-chitosans
Preparation, characterization, and antioxidant properties of gallic acid-grafted-chitosans
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DOI：
10.1016/j.carbpol.2010.10.019
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发表时间：
2011-02-01
影响因子：
11.2
通讯作者：
Je, Jae-Young
中科院分区：
文献类型：
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作者：
Cho, Young-Soak;Kim, Se-Kwon;Je, Jae-Young
为了提高没食子酸接枝壳聚糖的抗氧化性和水溶性，采用自由基接枝法制备了4种不同接枝率的没食子酸接枝壳聚糖。通过核磁共振氢谱和薄层色谱分析，证实了GA接枝到了壳聚糖上。GA-g-壳聚糖的抗氧化性能进行了评估，使用几个在体外模型。GA含量最高的GA g壳聚糖（I）在50 μ g/mL时对2，2-二苯基-1-苦肼的清除率为92.26%，对过氧化氢的清除率为93.15%。GA-g-壳聚糖（I）的还原能力也比其它几种壳聚糖高。所有的GA-g-壳聚糖显示出改善的抗氧化能力相比，普通壳聚糖在相同的条件下，没有没食子酸接枝处理。此外，GA-g-chitosans还表现出良好的细胞相容性，并有效地抑制细胞内活性氧（ROS）的形成在RAW 264. 7巨噬细胞的时间和剂量依赖性的方式。(C)2010爱思唯尔有限公司版权所有。
Gallic acid-grafted-chitosans (GA-g-chitosans) with four different grafting ratios were prepared by a free radical-induced grafting reaction in order to improve antioxidant and water-solubility. To verify the synthesis of GA-g-chitosans, H-1 NMR and thin layer chromatography were employed, and the results revealed that GA was grafted onto the chitosan. The antioxidant properties of the GA-g-chitosans were evaluated using several in vitro models. GA-g-chitosan (I), which has the highest GA content, showed 92.26% scavenging activity against 2,2-diphenyl-1-picrylhydrazyl and 93.15% hydrogen peroxide scavenging activity at 50 mu g/mL. GA-g-chitosan (I) was also showed higher reducing power compared to others. All GA-g-chitosans showed improved antioxidant capacities compared to plain chitosan treated in the same conditions without gallic acid grafting. Furthermore, the GA-g-chitosans also exhibited good cytocompatibility and effectively inhibited the formation of intracellular reactive oxygen species (ROS) in time- and dose-dependent manner in RAW264.7 macrophages. (C) 2010 Elsevier Ltd. All rights reserved.