Different roles for Aurora B in condensin targeting during mitosis and meiosis
Different roles for Aurora B in condensin targeting during mitosis and meiosis
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DOI：
10.1242/jcs.088336
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发表时间：
2011-11-01
影响因子：
4
通讯作者：
Csankovszki, Gyorgyi
中科院分区：
文献类型：
--
作者：
Collette, Karishma S.;Petty, Emily L.;Csankovszki, Gyorgyi
凝缩蛋白复合物是有丝分裂和减数分裂染色体分离所必需的。秀丽隐杆线虫，像其他后生动物一样，具有两种不同的有丝分裂和减数分裂凝缩蛋白复合物（I和II），它们占据不同的染色体结构域并执行非冗余功能。尽管有丝分裂和减数分裂染色体行为存在差异，但我们发现了这些过程中凝缩蛋白靶向的几个保守方面。在有丝分裂和减数分裂过程中，凝聚蛋白II在前期早期装载到染色体上，而凝聚蛋白I在进入前期中期装载。在有丝分裂和减数分裂过程中，凝缩蛋白I的定位，而不是凝缩蛋白II的定位，与线虫的染色体乘客激酶Aurora B （AIR-2）的定位非常相似。有趣的是，在有丝分裂后期，凝缩蛋白I和AIR-2也在纺锤体中间区共定位，在减数分裂后期，在分离的染色体之间共定位。一贯地，AIR-2影响凝缩蛋白I的靶向性，而不影响凝缩蛋白II。然而，在这些过程中，AIR-2在凝缩蛋白I靶向中所起的作用是不同的。在有丝分裂中，凝聚蛋白I的染色体结合需要AIR-2活性。相比之下，在减数分裂期间，凝聚蛋白I的染色体结合不需要AIR-2活性，但它为正确的空间靶向复合物提供了线索。
Condensin complexes are essential for mitotic and meiotic chromosome segregation. Caenorhabditis elegans, like other metazoans, has two distinct mitotic and meiotic condensin complexes (I and II), which occupy distinct chromosomal domains and perform non-redundant functions. Despite the differences in mitotic and meiotic chromosome behavior, we uncovered several conserved aspects of condensin targeting during these processes. During both mitosis and meiosis, condensin II loads onto chromosomes in early prophase, and condensin I loads at entry into prometaphase. During both mitosis and meiosis, the localization of condensin I, but not condensin II, closely parallels the localization of the chromosomal passenger kinase Aurora B (AIR-2 in C. elegans). Interestingly, condensin I and AIR-2 also colocalize on the spindle midzone during anaphase of mitosis, and between separating chromosomes during anaphase of meiosis. Consistently, AIR-2 affects the targeting of condensin I but not condensin II. However, the role AIR-2 plays in condensin I targeting during these processes is different. In mitosis, AIR-2 activity is required for chromosomal association of condensin I. By contrast, during meiosis, AIR-2 is not required for condensin I chromosomal association, but it provides cues for correct spatial targeting of the complex.