THE ROLE OF CD18-MEDIATED ADHESION IN NEUTROPHIL SEQUESTRATION INDUCED BY INFUSION OF ACTIVATED PLASMA IN RABBITS
THE ROLE OF CD18-MEDIATED ADHESION IN NEUTROPHIL SEQUESTRATION INDUCED BY INFUSION OF ACTIVATED PLASMA IN RABBITS
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DOI：
10.1165/ajrcmb/7.2.140
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发表时间：
1992-08-01
影响因子：
6.4
通讯作者：
DOERSCHUK, CM
中科院分区：
文献类型：
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作者：
DOERSCHUK, CM
输注激活的血浆可导致中性粒细胞减少和中性粒细胞在微血管内的隔离。这项研究考察了黏附糖蛋白复合体CD11/CD18在这种隔离中的作用。用抗CD18单抗(MAb)60.3或生理盐水预处理的兔，分别输注酵母多糖激活血浆(ZAP)或生理盐水。测定单抗60.3对ZAP引起的循环中性粒细胞计数、放射性标记的中性粒细胞在肺内的动力学变化以及中性粒细胞在肺内的微血管聚集的影响。结果表明，单抗60.3对中性粒细胞减少症的发生率和严重程度均无抑制作用，但可阻止持续的中性粒细胞减少症。此外，mAb60.3完全阻止了ZAP引起的中性粒细胞动力学改变，并在很大程度上抑制了中性粒细胞在毛细血管和小血管内的聚集。我们得出结论，中性粒细胞聚集是一个两步过程，第一步通过CD18非依赖的机制发生，可能涉及刺激诱导的中性粒细胞变形性降低，并减缓中性粒细胞通过肺的转运。第二步需要依赖CD18的黏附，并需要中性粒细胞在肺微血管内的长期聚集。
Infusion of activated plasma induces neutropenia and sequestration of neutrophils within the microvasculature. This study examined the role of the adhesion glycoprotein complex, CD11/CD18, in this sequestration. Rabbits pretreated with either the anti-CD18 monoclonal antibody (mAb) 60.3 or saline were given infusions of zymosan-activated plasma (ZAP) or saline. The effect of mAb 60.3 on the changes in circulating neutrophil counts, radiolabeled neutrophil kinetics in the lung, and the pulmonary microvascular accumulation of neutrophils induced by ZAP infusion was determined. The data show that pretreatment with mAb 60.3 did not inhibit either the rate of onset or the severity of the neutropenia but prevented the sustained neutropenia. In addition, mAb 60.3 completely prevented the ZAP-induced changes in radiolabeled neutrophil kinetics and largely inhibited the accumulation of neutrophils within the capillaries and the small vessels when evaluated after 15 min of ZAP infusion. We conclude that neutrophil accumulation is a two-step process, the first occurring through a CD18-independent mechanism that may involve a stimulus-induced decrease in neutrophil deformability and acts to slow neutrophil transit through the lung. The second step requires CD18-dependent adhesion and is needed for prolonged accumulation of neutrophils within the pulmonary microvasculature.