Taste responses to sweet stimuli in α-gustducin knockout and wild-type mice
Taste responses to sweet stimuli in α-gustducin knockout and wild-type mice
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DOI：
10.1093/chemse/bjj062
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发表时间：
2006-07-01
期刊：
影响因子：
3.5
通讯作者：
Hellekant, Goeran
中科院分区：
文献类型：
--
作者：
Danilova, Vicktoria;Damak, Sami;Hellekant, Goeran
α-味觉蛋白在甜味传导中的重要性是基于用蔗糖和人工甜味剂SC 45647获得的数据。在这里，我们研究了alpha-gustducin在甜味中的作用。我们比较了α-味觉蛋白基因敲除（KO）和野生型（WT）小鼠对11种不同甜味剂的行为和电生理反应，这些甜味剂代表碳水化合物、人工甜味剂和甜味氨基酸。在行为实验中，超过48小时的偏好率在两瓶偏好测试中测量。在电生理实验中，记录鼓索（CT）和舌咽（NG）神经的综合反应。我们发现KO小鼠对安赛蜜、甘精素、果糖、NC 00174、D-苯丙氨酸、L-脯氨酸、D-色氨酸、糖精、SC 45647、蔗糖的偏好率显着低于WT，但纽甜的偏好率不低于WT。除纽甜外，KO小鼠对所有甜味剂的神经反应均小于WT小鼠。WT和KO小鼠中的反应之间的差异在CT中比在NG中更明显。这些数据表明，α-味觉蛋白一般参与甜味的转导。
The importance of alpha-gustducin in sweet taste transduction is based on data obtained with sucrose and the artificial sweetener SC45647. Here we studied the role of alpha-gustducin in sweet taste. We compared the behavioral and electrophysiological responses of alpha-gustducin knockout (KO) and wild-type (WT) mice to 11 different sweeteners, representing carbohydrates, artificial sweeteners, and sweet amino acids. In behavioral experiments, over 48-h preference ratios were measured in two-bottle preference tests. In electrophysiological experiments, integrated responses of chorda tympani (CT) and glossopharyngeal (NG) nerves were recorded. We found that preference ratios of the KO mice were significantly lower than those of WT for acesulfame-K, dulcin, fructose, NC00174, D-phenylalanine, L-proline, D-tryptophan, saccharin, SC45647, sucrose, but not neotame. The nerve responses to all sweeteners, except neotame, were smaller in the KO mice than in the WT mice. The differences between the responses in WT and KO mice were more pronounced in the CT than in the NG. These data indicate that alpha-gustducin participates in the transduction of the sweet taste in general.