Two Novel Flavin-Containing Monooxygenases Involved in Biosynthesis of Aliphatic Glucosinolates.
Two Novel Flavin-Containing Monooxygenases Involved in Biosynthesis of Aliphatic Glucosinolates.
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参与脂肪族芥子油苷生物合成的两种新型含黄素单加氧酶
DOI：
10.3389/fpls.2016.01292
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发表时间：
2016
影响因子：
5.6
通讯作者：
Ji W
中科院分区：
文献类型：
--
作者：
Kong W;Li J;Yu Q;Cang W;Xu R;Wang Y;Ji W
硫代葡萄糖苷是一类来自十字花科植物的次生代谢物，来源于氨基酸，具有多种生物活性，如在生物防御中，依赖于其侧链的修饰。脂肪族(蛋氨酸衍生)硫代葡萄糖苷合成的第一步结构修饰--S--将甲硫基烷基硫代葡萄糖苷氧化成甲硫基烷基硫代葡萄糖苷--被发现是由五种含黄素的单加氧酶(FMOS-OX1-5)催化的。在这里，我们报告了另外两种FMOGS-OX酶，FMOGS-OX6和FMOGS-OX7，分别由At1g12130和At1g12160编码。FMOGS-OX6和FMOGS-OX7的过表达降低了甲硫烷基硫代葡萄糖苷与甲硫烷基硫代硫代葡萄糖苷之和的比例，这表明这两个基因的导入将甲硫烷基硫代硫代葡萄糖苷转化为甲硫基烷基硫代葡萄糖苷。表达谱分析表明，这两个基因的空间表达非常相似，并且与其他主要在维管组织中表达的FMOGS-ox基因部分重叠。我们进一步分析了所有7个FMOGS-ox基因对外源脱落酸、1-氨基环丙烷-1-羧酸(ACC)、茉莉酸(JA)、水杨酸、吲哚-3-乙酸(IAA)以及低温和高温处理的响应表达模式。虽然这些基因对刺激的变化表现出相同的趋势，但每个基因的敏感度却有很大的不同。当对环境刺激作出反应时，FMOGS-OX基因在空间表达上的变化表明硫代葡萄糖苷修饰是一个复杂和微调的调节。这两个新的FMOGS-OX酶的鉴定将加强对硫代葡萄糖苷修饰的理解和这些重复基因进化的重要性。
Glucosinolates, a class of secondary metabolites from cruciferous plants, are derived from amino acids and have diverse biological activities, such as in biotic defense, depending on their side chain modification. The first structural modification step in the synthesis of aliphatic (methionine-derived) glucosinolates—S-oxygenation of methylthioalkyl glucosinolates to methylsulfinylalkyl glucosinolates—was found to be catalyzed by five flavin-containing monooxygenases (FMOs), FMOGS-OX1-5. Here, we report two additional FMOGS-OX enzymes, FMOGS-OX6, and FMOGS-OX7, encoded by At1g12130 and At1g12160, respectively. The overexpression of both FMOGS-OX6 and FMOGS-OX7 decreased the ratio of methylthioalkyl glucosinolates to the sum of methylthioalkyl and methylsulfinylalkyl glucosinolates, suggesting that the introduction of the two genes converted methylthioalkyl glucosinolates into methylsulfinylalkyl glucosinolates. Analysis of expression pattern revealed that the spatial expression of the two genes is quite similar and partially overlapped with the other FMOGS-OX genes, which are primarily expressed in vascular tissue. We further analyzed the responsive expression pattern of all the seven FMOGS-OX genes to exogenous treatment with abscisic acid, 1-aminocyclopropane-1-carboxylic acid (ACC), jasmonic acid (JA), salicylic acid, indole-3-acetic acid (IAA), and low and high temperatures. Although these genes showed same tendency toward the changing stimulus, the sensitivity of each gene was quite different. The variety in spatial expression among the FMOGS-OX genes while responding to environmental stimulus indicated a complex and finely tuned regulation of glucosinolates modifications. Identification of these two novel FMOGS-OX enzymes will enhance the understanding of glucosinolates modifications and the importance of evolution of these duplicated genes.
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影响因子：
10.7
作者：
Hanada K;Sawada Y;Kuromori T;Klausnitzer R;Saito K;Toyoda T;Shinozaki K;Li WH;Hirai MY
通讯作者：
Hirai MY
影响因子：
5.6
作者：
Del Carmen Martínez-Ballesta M;Moreno DA;Carvajal M
通讯作者：
Carvajal M
影响因子：
7.2
作者：
Hansen, Bjarne G.;Kliebenstein, Daniel J.;Halkier, Barbara A.
通讯作者：
Halkier, Barbara A.
影响因子：
6.9
作者：
Li, Jing;Kristiansen, Kim A.;Halkier, Barbara A.
通讯作者：
Halkier, Barbara A.
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF