Single crystals of large ribosomal particles from Halobacterium marismortui diffract to 6 A.
Single crystals of large ribosomal particles from Halobacterium marismortui diffract to 6 A.
复制标题
来自海生盐杆菌的大核糖体颗粒的单晶衍射至 6 A。
DOI：
10.1016/0022-2836(87)90362-7
复制
发表时间：
1987
影响因子：
5.6
通讯作者：
Yonath,A
中科院分区：
文献类型：
--
作者：
Makowski,I;Frolow,F;Saper,MA;Shoham,M;Wittmann,HG;Yonath,A
通过接种获得了海死盐杆菌50 S核糖体亚基的大的、有序的三维晶体。晶体经同步辐射X射线衍射表征为单斜晶系，空间群P2 1，晶胞参数a= 182（±5）A，B= 584（±10）A，c= 186（±5）A，β= 109°。在4° C时，晶体（0.6 mm× 0.6 mm× 0.1 mm）的分辨率为6 μ m，并且在同步加速器光束中稳定数小时。紧密堆积反映在晶体学晶胞参数和电子显微照片的正染色薄切片的嵌入晶体
Large, well-ordered three-dimensional crystals of 50 S ribosomal subunits from Halobacterium marismortui have been obtained by seeding. The crystals have been characterized with synchrotron X-ray radiation as monoclinic, space group P2 1, with unit cell dimensions of a= 182 (±5) A ̊, b= 584 (±10) A ̊, c= 186 (±5) A ̊, β= 109°. At 4° C, the crystals (0.6 mm× 0.6 mm× 0.1 mm) diffract to 6 Å resolution and are stable in the synchrotron beam for several hours. Compact packing is reflected from the crystallographic unit cell parameters and from electron micrographs of positively stained thin sections of embedded crystals