Prevalence and genotyping of Giardia duodenalis from beef calves in Alberta, Canada
Prevalence and genotyping of Giardia duodenalis from beef calves in Alberta, Canada
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DOI：
10.1016/s0304-4017(02)00422-3
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发表时间：
2003-03-25
影响因子：
2.6
通讯作者：
Olson, ME
中科院分区：
文献类型：
--
作者：
Appelbee, AJ;Frederick, LM;Olson, ME
家畜贾第鞭毛虫感染受到越来越多的关注，因为粪便分布在田地和牧场径流中可能污染地表和地下沃茨。本研究的目的是确定加拿大阿尔伯塔省主要肉牛养殖场肉牛中贾第鞭毛虫的患病率和基因型。粪便样品收集自阿尔伯塔9个农场2-10周龄的肉牛。检查样品中是否存在G。尿道囊肿免疫荧光染色。在495份粪便样本中，有168份发现了贾第虫包囊，农场中的患病率为7 - 60%。利用PCR和16 S-rRNA基因座的292 bp片段的测序的阳性分离株的基因型分析，揭示了42株中的41个有蹄类家畜基因型。一个分离株与组合A基因型相同。本研究的结果表明，该地区的肉牛主要感染家畜基因型，该基因型被认为是偶蹄动物宿主特有的，对人类无感染性。这表明肉牛携带的贾第虫可能是最小的人畜共患病威胁。(C)2003 Elsevier Science B. V.保留所有权利。
Giardia infections in domestic cattle has come under increasing scrutiny owing to the potential contamination of surface and ground waters through manure distribution on fields and pasture runoff. The objective of the study was to determine the prevalence and genotypes of Giardia duodenalis in beef calves in major beef cow calf farms in Alberta, Canada. Fecal samples were collected from beef calves aged 2-10 weeks at nine farms in Alberta. Samples were examined for the presence of G. duodenalis cysts by immunofluorescent staining. Giardia cysts were found in 168 of the 495 fecal samples examined, with prevalence ranging from 7 to 60% among farms. Genotypic analysis of positive isolates utilizing PCR and sequencing of a 292 bp fragment of the 16S-rRNA locus, revealed the hoofed livestock genotype in 41 of the 42 isolates. One isolate was identical to the Assemblage A genotype. The results of this study demonstrate that beef calves in this area are primarily infected with the livestock genotype which is thought to be specific to artiodactyl hosts and non-infective to humans. This suggests that the Giardia carried by beef cattle may be a minimal zoonotic threat. (C) 2003 Elsevier Science B.V. All rights reserved.