Comparative analysis of plant and animal models for characterization of Burkholdetia cepacia virulence
Comparative analysis of plant and animal models for characterization of Burkholdetia cepacia virulence
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DOI：
10.1128/iai.71.9.5306-5313.2003
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发表时间：
2003-09-01
影响因子：
3.1
通讯作者：
Sokol, PA
中科院分区：
文献类型：
--
作者：
Bernier, SP;Silo-Suh, L;Sokol, PA
开发了一种简单的苜蓿模型作为洋葱伯克霍尔德菌复合体毒力研究的替代感染模型。在接种 10(1) 至 10(5) CFU 的大多数洋葱伯克酵母复合体菌株后 7 天内，在受伤的苜蓿幼苗中观察到疾病症状。在苜蓿模型中测试了来自洋葱伯克霍尔德杆菌复合体的七个基因变种的菌株的毒力，并且属于同一基因变种的菌株的毒力程度通常相似。多食伯克霍尔德菌菌株和稳定伯克霍尔德菌菌株不会引起苜蓿幼苗的疾病症状。还使用大鼠琼脂珠模型测试了代表性菌株的毒力。大多数测试的菌株能够引起慢性肺部感染； B. stableis 菌株是例外。大多数在苜蓿感染模型中具有毒性的菌株在肺部感染模型中也具有毒性。洋葱伯克霍尔德杆菌基因变种 III 突变体 K56pvdA::tp 和 K56-H15 在苜蓿感染模型中的毒性显着低于其亲本菌株。因此，这种苜蓿感染模型可能是评估洋葱伯克霍尔德杆菌复合体菌株毒力和鉴定新毒力相关基因的有用工具。
A simple alfalfa model was developed as an alternative infection model for virulence studies of the Burkholderia cepacia complex. Symptoms of disease were observed in wounded alfalfa seedlings within 7 days following inoculation of 10(1) to 10(5) CFU of most strains of the B. cepacia complex. Strains from seven genomovars of the B. cepacia complex were tested for virulence in the alfalfa model, and the degree of virulence was generally similar in strains belonging to the same genomovar. Strains of Burkholderia multivorans and some strains of Burkholderia stabilis did not cause symptoms of disease in alfalfa seedlings. Representative strains were also tested for virulence using the rat agar bead model. Most of the strains tested were able to establish chronic lung infections; B. stabilis strains were the exception. Most of the strains that were virulent in the alfalfa infection model were also virulent in the lung infection model. The B. cepacia genomovar III mutants K56pvdA::tp and K56-H15 were significantly less virulent in the alfalfa infection model than their parent strain. Therefore, this alfalfa infection model may be a useful tool for assessing virulence of strains of the B. cepacia complex and identifying new virulence-associated genes.