SELECTIVE AND DIFFERENTIAL BINDING OF INTERLEUKIN (IL)-1-ALPHA, IL-1-BETA, IL-2 AND IL-6 TO GLYCOSAMINOGLYCANS
SELECTIVE AND DIFFERENTIAL BINDING OF INTERLEUKIN (IL)-1-ALPHA, IL-1-BETA, IL-2 AND IL-6 TO GLYCOSAMINOGLYCANS
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DOI：
10.1002/eji.1830221139
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发表时间：
1992-11-01
影响因子：
5.4
通讯作者：
RIDER, CC
中科院分区：
文献类型：
--
作者：
RAMSDEN, L;RIDER, CC
用放射性碘标记的重组白细胞介素（IL）-1 α、IL-1 β、IL-2和IL-6在固定化多糖柱上的亲和层析研究了IL-1 α、IL-1 β、IL-2和IL-6与酸性多糖的结合。每种白细胞介素均表现出选择性结合保留。总的来说，肝素显著结合所有四种白细胞介素，而硫酸软骨素几乎没有保留。IL-1 α和IL-1 β表现出不同的结合，只有后者与透明质酸结合。IL-2几乎完全保留在岩藻依聚糖上。非碘化重组IL-2与岩藻依聚糖的结合类似，并且在采用IL-2依赖性CTLL细胞的生物测定中发现岩藻依聚糖螯合IL-2活性。在所有其他测试的情况下，白细胞介素保留是部分的，这意味着白细胞介素结合位点沿多糖链沿着稀疏分布。这些发现表明，在免疫应答期间，白细胞介素将倾向于通过与细胞外基质和细胞表面上的糖胺聚糖相互作用而保留在分泌位点。
The binding of interleukin (IL)-1alpha, IL-1beta, IL-2 and IL-6 to acidic polysaccharides was investigated by affinity chromatography of the recombinant, radioiodinated interleukins on columns of immobilized polysaccharide. Each interleukin showed selective binding retention. Overall heparin bound all four interleukins significantly, whereas chondroitin sulfate provided little retention. IL-1alpha and IL-1beta showed differential binding, with only the latter binding to hyaluronic acid. IL-2 was virtually completely retained on fucoidan. Noniodinated recombinant IL-2 bound similarly to fucoidan, and fucoidan was found to sequester IL-2 activity in a bioassay employing IL-2-dependent CTLL cells. In all other cases tested, interleukin retention was partial, implying that interleukin binding sites are sparsely distributed along the polysaccharide chains. These findings suggest that during the immune response, interleukins will tend to be retained at sites of secretion by interaction with glycosaminoglycans in the extracellular matrix and on cell surfaces.