CONTINUOUS RECORDING OF PHOTOCHEMICAL AND NONPHOTOCHEMICAL CHLOROPHYLL FLUORESCENCE QUENCHING WITH A NEW TYPE OF MODULATION FLUOROMETER
CONTINUOUS RECORDING OF PHOTOCHEMICAL AND NONPHOTOCHEMICAL CHLOROPHYLL FLUORESCENCE QUENCHING WITH A NEW TYPE OF MODULATION FLUOROMETER
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DOI：
10.1007/bf00024185
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发表时间：
1986-01-01
影响因子：
3.7
通讯作者：
BILGER, W
中科院分区：
文献类型：
--
作者：
SCHREIBER, U;SCHLIWA, U;BILGER, W
本文介绍了一种新研制的荧光测量系统，用于记录叶绿素荧光诱导动力学（考茨基效应）和连续测定荧光猝灭的光化学和非光化学成分。基于脉冲调制原理的测量系统选择性地监测弱测量光束的荧光产率，并且即使受到极高强度的光化光的影响也不受影响。通过重复应用饱和强度的短光脉冲，重复记录完全抑制光化学猝灭时的荧光产率，从而可以确定光化学猝灭和非光化学猝灭的连续图。将这些图与不同光化照明强度下可变荧光的时间过程进行比较。所观察到的动力学之间的差异进行了讨论。它示出的调制荧光计，结合饱和光脉冲的应用，提供了必要的信息超出了与传统的叶绿素荧光计获得。
A newly developed fluorescence measuring system is employed for the recording of chlorophyll fluorescence induction kinetics (Kautsky-effect) and for the continuous determination of the photochemical and non-photochemical components of fluorescence quenching. The measuring system, which is based on a pulse modulation principle, selectively monitors the fluorescence yield of a weak measuring beam and is not affected even by extremely high intensities of actinic light. By repetitive application of short light pulses of saturating intensity, the fluorescence yield at complete suppression of photochemical quenching is repetitively recorded, allowing the determination of continuous plots of photochemical quenching and non-photochemical quenching. Such plots are compared with the time courses of variable fluorescence at different intensities of actinic illumination. The differences between the observed kinetics are discussed. It is shown that the modulation fluorometer, in combination with the application of saturating light pulses, provides essential information beyond that obtained with conventional chlorophyll fluorometers.