WGE: a CRISPR database for genome engineering.
WGE: a CRISPR database for genome engineering.
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DOI：
10.1093/bioinformatics/btv308
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发表时间：
2015-09-15
期刊：
影响因子：
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通讯作者：
Iyer V
中科院分区：
文献类型：
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作者：
Hodgkins A;Farne A;Perera S;Grego T;Parry-Smith DJ;Skarnes WC;Iyer V
总结：CRISPR-Cas9介导的基因组编辑技术的快速发展已经产生了许多在线和独立的工具来寻找和评分整个基因组的CRISPR位点。在这里，我们描述了Wellcome Trust桑格研究所基因组编辑数据库（WGE），该数据库使用新方法在基因组浏览器环境中计算，可视化和选择最佳CRISPR位点。WGE数据库目前存储了单个和成对的CRISPR位点以及位于小鼠和人类外显子组中的CRISPR的预先计算的脱靶信息。脱靶位点的评分和显示简单且直观，并且可以应用过滤器快速识别高质量的CRISPR位点。WGE还提供了一个工具，用于在同一个基因组浏览器中设计和展示基因靶向载体，沿着基因模型，蛋白质翻译和变异轨迹。WGE是开放的，可扩展的，可以设置为计算和呈现任何基因组的CRISPR位点。可用性和实施：WGE数据库可在www.sanger.ac.uk/htgt/wge上免费获得，联系方式：vvi@sanger.ac.uk或skarnes@sanger.ac.uk补充信息：补充数据可在生物信息学在线获得。
Summary: The rapid development of CRISPR-Cas9 mediated genome editing techniques has given rise to a number of online and stand-alone tools to find and score CRISPR sites for whole genomes. Here we describe the Wellcome Trust Sanger Institute Genome Editing database (WGE), which uses novel methods to compute, visualize and select optimal CRISPR sites in a genome browser environment. The WGE database currently stores single and paired CRISPR sites and pre-calculated off-target information for CRISPRs located in the mouse and human exomes. Scoring and display of off-target sites is simple, and intuitive, and filters can be applied to identify high-quality CRISPR sites rapidly. WGE also provides a tool for the design and display of gene targeting vectors in the same genome browser, along with gene models, protein translation and variation tracks. WGE is open, extensible and can be set up to compute and present CRISPR sites for any genome. Availability and implementation: The WGE database is freely available at www.sanger.ac.uk/htgt/wge Contact: vvi@sanger.ac.uk or skarnes@sanger.ac.uk Supplementary information: Supplementary data are available at Bioinformatics online.