Green leaf volatile-burst in Arabidopsis is governed by galactolipid oxygenation by a lipoxygenase that is under control of calcium ion
Green leaf volatile-burst in Arabidopsis is governed by galactolipid oxygenation by a lipoxygenase that is under control of calcium ion
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DOI：
10.1016/j.bbrc.2018.10.042
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发表时间：
2018-11-02
影响因子：
3.1
通讯作者：
Matsui, Kenji
中科院分区：
文献类型：
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作者：
Mochizuki, Satoshi;Matsui, Kenji
植物在受到草食动物伤害等造成组织破坏后，几乎立即形成绿叶挥发物(GLV)。这种快速形成的GLV，即GLV-Burst，是植物依赖GLV的直接和间接防御的重要因素。然而，GLV-Burst的发病机制尚不清楚。我们观察到，拟南芥脂肪氧合酶2(AtLOX2)形成的单半乳糖二酰甘油过氧化氢(MGDG-OOHs)控制着拟南芥的GLV-Burst。在组织破裂过程中加入选择性钙离子螯合剂BAPTA，有效地抑制了MGDG-OOHS和GLV-Burst的形成。Ca~(2+)的加入解除了这种抑制作用。因此，我们认为，在白三烯的形成中观察到的那样，依赖于钙的AtLOX2的激活促进了GLV的形成，这是通过依赖于钙的动物细胞中的LOXs的激活来调节的。(C)2018 Elsevier Inc.保留所有权利。
Plants form green leaf volatiles (GLVs) almost instantly after tissue disruption caused by damages, such as herbivore damage. This rapid formation of GLVs, namely GLV-burst, is an essential factor for the plants' GLV-dependent direct and indirect defenses. However, mechanism of GLV-burst remains unknown. We observed that the formation of monogalactosyldiacylglycerol hydroperoxides (MGDG-OOHs) by Arabidopsis lipoxygenase 2 (AtLOX2) governs GLV-burst in Arabidopsis. Addition of a Ca2+ selective chelating reagent, BAPTA, during tissue disruption effectively suppressed the formation of MGDG-OOHS as well as GLV-burst. This suppression was relieved by the addition of Ca2+. Therefore, we propose that Ca2+-dependent activation of AtLOX2 facilitates GLV-burst formation as observed in leukotriene formation, which is regulated by Ca2+-dependent activation of LOXs in animal cells. (C) 2018 Elsevier Inc. All rights reserved.