CHARACTERIZATION AND AUTORADIOGRAPHIC LOCALIZATION OF HISTAMINE H1 RECEPTORS IN HUMAN NASAL TURBINATES
CHARACTERIZATION AND AUTORADIOGRAPHIC LOCALIZATION OF HISTAMINE H1 RECEPTORS IN HUMAN NASAL TURBINATES
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DOI：
10.1016/0091-6749(92)90298-g
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发表时间：
1992-06-01
影响因子：
14.2
通讯作者：
KALINER, MA
中科院分区：
文献类型：
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作者：
OKAYAMA, M;BARANIUK, JN;KALINER, MA
为探讨组胺H-1受体（H1 R）在人鼻粘膜的定位，用放射自显影法研究了H1 R在人下鼻甲的分布。在饱和结合研究中，将冷冻切片与不同浓度的[H-3]吡拉明孵育，并与1 nmol/L的[H-3]吡拉明孵育用于放射自显影。通过加入2 μ mol/L的吡拉明测定非特异性结合。Scatchard分析显示了高亲和力结合位点，H1 R的最大结合容量为193 +/- 46 fmol/mg蛋白质，解离常数为0.6 +/- 0.1 nmol/L。放射自显影显示H1 R仅存在于血管内皮上。在粘膜下腺体或上皮中未观察到特异性标记。这些结果扩展并支持了我们先前的发现，即组胺通过H1 R直接引起血管通透性，并通过反射间接刺激鼻腺分泌。
To examine the localization of histamine H-1 receptors (H1R) in human nasal mucosa, the autoradiographic distribution of H1R was studied in human nasal inferior turbinates. Cryostat sections were incubated with various concentration of [H-3]pyrilamine in saturation-binding studies and with 1 nmol/L of [H-3]pyrilamine for autoradiography. Nonspecific binding was determined by adding 2-mu-mol/L of pyrilamine. Scatchard analysis demonstrated high-affinity binding sites with a maximum binding capacity of H1R of 193 +/- 46 fmol/mg of protein, and dissociation constant was 0.6 +/- 0.1 nmol/L. Autoradiograms indicated H1R exist exclusively on the endothelium of vessels. No specific labeling could be observed in the submucosal glands or epithelium. These results extend and support our previous finding that histamine directly causes vascular permeability through H1R and stimulates nasal glandular secretion indirectly through reflexes.