Muscle from aged rats is resistant to mechanotherapy during atrophy and reloading
Muscle from aged rats is resistant to mechanotherapy during atrophy and reloading
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DOI：
10.1007/s11357-020-00215-y
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发表时间：
2020-06-25
期刊：
影响因子：
5.6
通讯作者：
Dupont-Versteegden, Esther E.
中科院分区：
文献类型：
--
作者：
Lawrence, Marcus M.;Van Pelt, Douglas W.;Dupont-Versteegden, Esther E.
按摩是一种可行的机械疗法，可以改善废用性萎缩期间的蛋白质周转，并改善成人肌肉废用性萎缩恢复期间的肌肉再生。因此，我们研究了按摩是否能使老年大鼠在正常负重（WB）、后肢悬吊（HS）或后肢悬吊（RE）状态下的骨骼肌产生有益的适应性。将30月龄雄性Fischer 344/Brown Norway大鼠分为2个实验：(1)WB组7天（WB,n = 8）、WB组加按摩组（WBM,n = 8）、HS组7天（HS7,n = 8）或HS组加按摩组（HSM,n = 8）； (2) WB组14天（WB14,n = 8）、HS组14天（HS14,n = 8）、重装组（RE,n = 10）或重装组按摩组（REM,n = 10）。使用氧化氘（D2O）标记来评估各组的动态蛋白质和核糖体周转，并评估合成代谢信号通路。在RE或WB期间，按摩确实对合成代谢有益。相比之下，与HS相比，HS期间的按摩增强了按摩肢体和对侧非按摩肢体的肌原纤维蛋白周转，但这并不能防止肌肉损失。总的来说，数据表明，按摩并不是一种有效的机械疗法，可以预防老年大鼠肌肉失用期间的萎缩或重新加载期间肌肉质量的恢复。
Massage is a viable mechanotherapy to improve protein turnover during disuse atrophy and improve muscle regrowth during recovery from disuse atrophy in adult muscle. Therefore, we investigated whether massage can cause beneficial adaptations in skeletal muscle from aged rats during normal weight-bearing (WB) conditions, hindlimb suspension (HS), or reloading (RE) following HS. Aged (30 months) male Fischer 344/Brown Norway rats were divided into two experiments: (1) WB for 7 days (WB,n = 8), WB with massage (WBM,n = 8), HS for 7 days (HS7,n = 8), or HS with massage (HSM,n = 8), and (2) WB for 14 days (WB14,n = 8), HS for 14 days (HS14,n = 8), reloading (RE,n = 10), or reloading with massage (REM,n = 10) for 7 days following HS. Deuterium oxide (D2O) labeling was used to assess dynamic protein and ribosome turnover in each group and anabolic signaling pathways were assessed. Massage did have an anabolic benefit during RE or WB. In contrast, massage during HS enhanced myofibrillar protein turnover in both the massaged limb and contralateral non-massaged limb compared with HS, but this did not prevent muscle loss. Overall, the data demonstrate that massage is not an effective mechanotherapy for prevention of atrophy during muscle disuse or recovery of muscle mass during reloading in aged rats.