Cytokine expression in the alkali-burned cornea
Cytokine expression in the alkali-burned cornea
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DOI：
10.1076/ceyr.16.7.670.5057
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发表时间：
1997-07-01
影响因子：
2
通讯作者：
Kinoshita, S
中科院分区：
文献类型：
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作者：
Sotozono, C;He, JC;Kinoshita, S
目的.本研究旨在探讨碱烧伤小鼠角膜组织中细胞因子的表达谱，以阐明碱烧伤后角膜损伤与修复的机制。用聚合酶链反应（RT-PCR）鉴定碱烧伤角膜中细胞因子的表达，然后用ELISA定量。根据ELISA结果，进行免疫组织化学分析以定位细胞因子的表达。在检测的10种细胞因子中，IL-1（IL-1 β）、IL-6、IL-10和TNF-α mRNA在碱烧伤角膜中表达。定量显示，IL-1 α和IL-6在碱烧伤的早期被强烈诱导，IL-1 α（53.2 pg/角膜）和IL-6（23.6 pg/角膜）的峰值产生分别出现在第3天和第7天。在烧伤后42天内没有显著升高。免疫组化分析显示IL-1 α和IL-6主要定位于再生上皮基底细胞。角膜中的IL-1 α和IL-6水平在碱破裂的早期阶段在再生上皮中显著升高，并且可能在相关的角膜损伤和修复中起重要作用。
Purpose. This study investigated the cytokine expression profile in alkali-burned mouse corneas, in order to elucidate the mechanisms of corneal damage and repair.Methods. The cytokines expressed in alkali-burned corneas were identified by polymerase chain reaction (RT-PCR), then quantitated using ELISA. Based on the ELISA results, immunohistochemical analyses were performed to localize cytokine expression.Results. Among the ten cytokines examined, IL-1 (IL-1 beta), IL-6, IL-10 and TNF-alpha mRNA were expressed in alkali-burned corneas. Quantitation revealed that IL-1 alpha and IL-6 were strongly induced in the early stages of alkali burn, peak production of IL-1 alpha (53.2 pg/cornea) and IL-6 (23.6 pg/cornea) occurring at days 3 and 7, respectively, The production of IL-10 and TNF-alpha. was not significantly elevated during the 42 day period after burn. Immunohistochemical analyses revealed that both IL-1 alpha and IL-6 were mainly localized in regenerating epithelial basal cells.Conclusions. IL-1 alpha and IL-6 levels in the cornea are dramatically elevated in the regenerated epithelium during the early stages of alkali bust, and may play an important role in associated corneal damage and repair.