Peptide sequence analysis on quadrupole mass spectrometers
Peptide sequence analysis on quadrupole mass spectrometers
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DOI：
10.1006/meth.1994.1029
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发表时间：
1994-01-01
期刊：
影响因子：
--
通讯作者：
Yates, John R., III
中科院分区：
文献类型：
--
作者：
McCormack, Ashley L.;Eng, Jimmy K.;Yates, John R., III
氨基酸序列分析的肽，在皮摩尔水平，通过三重四极杆质谱描述。讨论了微柱反相高效液相色谱-电喷雾电离串联质谱法对复杂多肽混合物的常规分析，如大肠杆菌细胞裂解液的胰蛋白酶消化和主要组织相容性复合体（MHC） II类抗原。利用肽的低能碰撞激活解离光谱直接搜索蛋白质和基因序列数据库。
Amino acid sequence analysis of peptides, at the picomole level, by triple-quadrupole mass spectrometry is described. Routine analysis of complex mixtures of peptides, such as a tryptic digest of Escherichia coli cell lysate and major histocompatibility complex (MHC) class II antigens, by microcolumn reverse-phase high-performance liquid chromatography electrospray ionization tandem mass spectrometry is discussed. Low-energy collision-activated dissociation spectra of peptides are used to directly search protein and gene sequence databases.