FAD-Dependent Glucose Dehydrogenase Immobilization and Mediation Within a Naphthoquinone Redox Polymer.
FAD-Dependent Glucose Dehydrogenase Immobilization and Mediation Within a Naphthoquinone Redox Polymer.
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FAD 依赖性葡萄糖脱氢酶在萘醌氧化还原聚合物中的固定和介导。
DOI：
10.1007/978-1-4939-6499-4_15
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发表时间：
2017
影响因子：
--
通讯作者：
Ross D. Milton
中科院分区：
文献类型：
--
作者：
Ross D. Milton
电化学活性聚合物（氧化还原聚合物）是用于在电极表面同时固定酶和进行电子转移的有用工具，其还用于增加生物催化剂的局部浓度。从电化学（电势）和生物化学观点来看，所采用的氧化还原对的性质必须与目标生物催化剂相容。本章详细介绍了萘醌功能化的氧化还原聚合物（NQ-LPEI）的合成，该聚合物用于与黄素腺嘌呤二核苷酸依赖性葡萄糖脱氢酶（FAD-GDH）进行电子通信，产生酶生物阳极，能够在相对较低的相关电位下为葡萄糖氧化提供大的催化电流密度。
Electrochemically-active polymers (redox polymers) are useful tools for simultaneous immobilization and electron transfer of enzymes at electrode surfaces, which also serve to increase the localized concentration of the biocatalyst. The properties of the employed redox couple must be compatible with the target biocatalyst from both an electrochemical (potential) and biochemical standpoint. This chapter details the synthesis of a naphthoquinone-functionalized redox polymer (NQ-LPEI) that is used to immobilize and electronically communicate with flavin adenine dinucleotide-dependent glucose dehydrogenase (FAD-GDH), yielding an enzymatic bioanode that is able to deliver large catalytic current densities for glucose oxidation at a relatively low associated potential.