A Highly-Efficient 3d Dnazyme Motor for SensitiveBiosensing Analysis
A Highly-Efficient 3d Dnazyme Motor for SensitiveBiosensing Analysis
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用于灵敏生物传感分析的高效 3d Dnazyme 电机
DOI：
10.1016/j.talanta.2022.123683
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发表时间：
2022
期刊：
影响因子：
6.1
通讯作者：
Yaqin Chai
中科院分区：
文献类型：
--
作者：
Xia Zhong;Yunrui Li;Yuanyuan Chang;Ruo Yuan;Yaqin Chai
本文针对高效DNA酶扩增检测策略的需要，设计了一种新型的三维DNA酶马达作为生物传感器平台，实现对目标DNA的灵敏检测。该马达由靶向激活的DNAzyme纳米线和底物H1-Fc共同固定在Au@Fe3O4纳米粒子（Au@Fe3O4纳米粒子）表面组成，具有高的DNA反应物局部浓度和缩短DNAzyme与底物之间的距离以增强电化学信号的作用。与传统的DNAzyme动力机器相比，3D DNAzyme马达的靶激活DNAzyme纳米线具有更大的灵活性和更强的切割能力，无需麻烦的序列优化，克服了空间限制，同时与邻近和远处的底物H1-Fc相互作用，输出大量高信号响应的切割产物。因此，基于纳米颗粒定位DNA设计和DNAzyme纳米线的上述优点，所报道的3D DNAzyme马达巧妙地克服了传统DNAzyme扩增检测策略中存在的反应物浓度低、DNAzyme灵活性有限和DNAzyme摆动范围小等诸多缺陷，实现了对靶DNA的灵敏检测，检测限为1.7 fM，范围为5 fM ~ 50 nM。令人印象深刻的是，3D DNAzyme马达在这里提出了一种新的策略，以实现有效的DNAzyme信号放大，并提供了一个参考，在未来的各种和功能的3D DNA机器的组装。
Herein, driven by the need of highly-efficient DNAzyme-amplified detection strategy, a novel 3D DNAzyme motor was designed as a biosensor platform for realizing sensitive detection of target DNA. The 3D DNAzyme motor was composed of target-activated DNAzyme nanowires and substrates H1-Fc that co-immobilized on Au@Fe3O4nanoparticles (Au@Fe3O4NPS) surface, possessing high local concentration of DNA reactants and shortened distance between DNAzyme and substrates for enhancing electrochemical signal. Compared with traditional DNAzyme-powered machines, the target-activated DNAzyme nanowires of 3D DNAzyme motor had greater flexibility and more powerful cleavage capability without troublesome sequence optimization, which overcame the space limitation and simultaneously interacted with adjacent and distant substrates H1-Fc to output a large amount of cleavage products with high signal response. Therefore, on account of the above-mentioned merits of nanoparticles localization DNA design and DNAzyme nanowires, the reported 3D DNAzyme motor ingeniously overcame many defects existing in traditional DNAzyme-amplified detection strategies such as low reactants concentration, limited flexibility of DNAzyme and small DNAzyme swing range, realizing the sensitive detection of target DNA with a detection limit of 1.7 fM ranging from 5 fM to 50 nM. Impressively, the 3D DNAzyme motor here presented a new strategy to achieve effective DNAzyme signal amplification and provided a reference for the assembly of various and functional 3D DNA machines in the future.